Reactivity | Mouse |
Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
Preparation | The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC. |
Concentration | 0.5 |
Concentration Units | mg/ml |
Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. |
Applications | FC - Quality tested |
Application Notes | Additional reported applications (for the relevant formats) include: ELISA as capture antibody or detection antibody, stimulation of B cell proliferation, and immunohistochemistry of acetone-fixed frozen sections. |
Application References | 1. Sieckmann D, et al. 1991. Hybridoma 10:121. 2. Stall AM. 1996. Weir's Handbook of Experimental Immunology pp. 27. |
Description | This antibody was raised by immunization with IgMa mAb TC31. It specifically reacts with mouse IgM of Igh-Ca and the related haplotypes (c, f, g, h, and j). It has been reported that beads coated with MA-69 antibody can stimulate B cell proliferation. |
Storage | The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |