Clone | 581 |
Isotype | Mouse IgG1, κ |
Reactivity | Human, Cross-Reactivity: Cynomolgus |
Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
Preparation | The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin. |
Concentration | 0.5mg/ml |
Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. |
Applications | FC - Quality tested |
Application Notes | The 581 antibody recognizes the class III group epitope which is resistant to sialidase/glycolyprotease and chymopapain treatment. |
Application References | 1. Schlossman SF, et al. 1995. Leukocyte Typing V:White Cell Differentiation Antigen. New York:Oxford University Press. 2. Felschow DM, et al. 2001. Blood 97:3768. 3. Rudin CE, et al. 1997. Br. J. Haematol. 97:488. 4. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) |
Description | CD34, also known as gp105-120, is a type I monomeric sialomucin-like glycophosphoprotein with an approximate molecular weight of 105-120 kD. Selectively expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nervous tissue, CD34 is a commonly used marker to identify human hematopoietic stem/progenitor cells. According to the differential sensitivity to enzymatic cleavage, four groups of epitopes of CD34 have been described. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands. |
Storage | The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |