Purified anti-human Perforin_详细资料

产品信息

克隆性：多克隆保存条件：蓝冰规格： 100 ug

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Purified anti-human Perforin参数介绍>>>>

Clone dG9
Isotype Mouse IgG2b, κ
Reactivity Human, Cross-Reactivity: Bovine (Cow, Cattle); does not cross-react with mouse¹.
Immunogen Purified granules from the human lymphoma cell line
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation The antibody was purified by affinity chromatography.
Concentration 0.5mg/ml
Recommended Usage Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Applications ICFC - Quality tested
IP, IF, IHC - Reported in the literature
Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation, intracellular flow cytometric analysis and immunofluorescence microscopy^5,7, and immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections^1,4.
Application References
1. Hameed A, et al. 1992. Am. J. Pathol. 140:1025. (IHC)
2. Schaerli P, et al. 2004. J. Exp. Med. 199:1265.
3. Watanabe N, et al. 1997. Blood 90:3662.
4. Mauad T, et al. 2004. Pediatr. Pulmonol. 38:233. (IHC)
5. Barrat FJ, et al. 1999. P. Natl. Acad. Sci. USA 96:8645. (IF)
6. Chen H, et al. 2005. J. Immunol. 175:591.
7. Bryceson YT, et al. 2007. Blood doi:10.1182/blood-2007-02-074468. (IF)
8. Wood SM, et al. 2009. Blood 114:4117. PubMed
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Description
Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.
Storage The antibody solution should be stored undiluted at 4°C.

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Clone	dG9
Isotype	Mouse IgG2b, κ
Reactivity	Human, Cross-Reactivity: Bovine (Cow, Cattle); does not cross-react with mouse¹.
Immunogen	Purified granules from the human lymphoma cell line
Formulation	Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation	The antibody was purified by affinity chromatography.
Concentration	0.5mg/ml
Recommended Usage	Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Applications	ICFC - Quality tested IP, IF, IHC - Reported in the literature
Application Notes	Additional reported applications (for the relevant formats) include: immunoprecipitation, intracellular flow cytometric analysis and immunofluorescence microscopy^5,7, and immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections^1,4.
Application References	1. Hameed A, et al. 1992. Am. J. Pathol. 140:1025. (IHC) 2. Schaerli P, et al. 2004. J. Exp. Med. 199:1265. 3. Watanabe N, et al. 1997. Blood 90:3662. 4. Mauad T, et al. 2004. Pediatr. Pulmonol. 38:233. (IHC) 5. Barrat FJ, et al. 1999. P. Natl. Acad. Sci. USA 96:8645. (IF) 6. Chen H, et al. 2005. J. Immunol. 175:591. 7. Bryceson YT, et al. 2007. Blood doi:10.1182/blood-2007-02-074468. (IF) 8. Wood SM, et al. 2009. Blood 114:4117. PubMed "
Description	Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.
Storage	The antibody solution should be stored undiluted at 4°C.