Alexa Fluor® 488 anti-mouse/human CD282 (TLR2)_详细资料

产品信息

克隆性：多克隆保存条件：蓝冰规格： 100 ug

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Alexa Fluor® 488 anti-mouse/human CD282 (TLR2)参数介绍>>>>

Clone T2.5
Isotype Mouse IgG1, κ
Reactivity Mouse, Human
Immunogen Recombinant extracellular domain of murine TLR2
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
Concentration 0.5mg/ml
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Applications
FC - Quality tested
Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation¹, immunohistochemical staining of acetone-fixed frozen sections,and inhibition of TLR2-dependent cell activation in vitro and in vivo¹.
Application References
1. Meng G, et al. 2004. J. Clin. Invest. 113:1473.
Description
Toll-like receptors (TLRs) are type I transmembrane signaling receptors characterized by containing leucine-rich repeats (LRR) extracellular domain and IL-1 receptor like intracellular domain. TLRs are responsible for recognizing pathogen-associated molecule patterns (PAMPs). They are primary sensors of both innate and adaptive immune systems. To date a total of 12 TLRs have been indentified. Toll-like receptor 2 (TLR2), known as CD282, is one of the important receptors. It is expressed on monocytes/macrophages, dendritic cells, granulocytes, endothelial and epithelial cells. By association with TLR1 or TLR6 and CD36, TLR2 is central to the innate immune response to diverse set of pathogens, including several components of Gram-positive bacteria such as peptidoglycan, lipoteihoic acid (LTA), lipoarabinomanan, lipoproteins, as well as different LPS from certain Gram-negative bacteria, yeast, spirochete and fungi. The T2.5 antibody reacts with both mouse and human TLR2. It is able to inhibit cell activation through blocking the interaction of TLR2 with its ligands.
Storage The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

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Clone	T2.5
Isotype	Mouse IgG1, κ
Reactivity	Mouse, Human
Immunogen	Recombinant extracellular domain of murine TLR2
Formulation	Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation	The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
Concentration	0.5mg/ml
Recommended Usage	Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Applications	FC - Quality tested
Application Notes	Additional reported applications (for the relevant formats) include: immunoprecipitation¹, immunohistochemical staining of acetone-fixed frozen sections,and inhibition of TLR2-dependent cell activation in vitro and in vivo¹.
Application References	1. Meng G, et al. 2004. J. Clin. Invest. 113:1473.
Description	Toll-like receptors (TLRs) are type I transmembrane signaling receptors characterized by containing leucine-rich repeats (LRR) extracellular domain and IL-1 receptor like intracellular domain. TLRs are responsible for recognizing pathogen-associated molecule patterns (PAMPs). They are primary sensors of both innate and adaptive immune systems. To date a total of 12 TLRs have been indentified. Toll-like receptor 2 (TLR2), known as CD282, is one of the important receptors. It is expressed on monocytes/macrophages, dendritic cells, granulocytes, endothelial and epithelial cells. By association with TLR1 or TLR6 and CD36, TLR2 is central to the innate immune response to diverse set of pathogens, including several components of Gram-positive bacteria such as peptidoglycan, lipoteihoic acid (LTA), lipoarabinomanan, lipoproteins, as well as different LPS from certain Gram-negative bacteria, yeast, spirochete and fungi. The T2.5 antibody reacts with both mouse and human TLR2. It is able to inhibit cell activation through blocking the interaction of TLR2 with its ligands.
Storage	The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.