PerCP anti-mouse CD45.1_详细资料

产品信息

克隆性：多克隆保存条件：蓝冰规格： 100 ug

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PerCP anti-mouse CD45.1参数介绍>>>>

Clone A20
Isotype Mouse (A.SW) IgG2a, κ
Reactivity Mouse
Immunogen SJL mouse thymocytes and splenocytes
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation The antibody was purified by affinity chromatography, and conjugated with PerCP under optimal conditions. The solution is free of unconjugated PerCP and unconjugated antibody.
Concentration 0.2mg/ml
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.
Applications
FC - Quality tested
Application Notes
The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation³, in vitro blocking of B cell responses^1,2, immunohistochemical staining of frozen sections: OCT embedded⁷ and acetone-fixed^4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)), and immunofluorescence microscopy⁹.
Application References
1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
3. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
4. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
5. Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC)
6. Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC)
7. Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC)
8. Pascal V, et al. 2007. J. Immunol. 179:1751. (FC)
9. Mende I, et al. 2006. Blood 107:1383. (IF, IHC, FC)
10. Phan TG, et al. 2007. Nature Immunol. 8:992. (FC)
11. Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed
12. Pascal V, et al.2007. J. Immunol. 179:1751. PubMed
13. Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed
14. Takada K, et al. 2009. J. Exp. Med. 206:2253. PubMed
15. Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed
16. Li LX, et al. 2010. J. Immunol. 184:1728. PubMed
17. Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed
18. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
19. Ninjik A, et al. 2012. J Immunol. 189:102. PubMed.
"
Description
CD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (e.g., RIII, SJL/J, STS/A, DA). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell types. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
"
Storage The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

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Clone	A20
Isotype	Mouse (A.SW) IgG2a, κ
Reactivity	Mouse
Immunogen	SJL mouse thymocytes and splenocytes
Formulation	Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation	The antibody was purified by affinity chromatography, and conjugated with PerCP under optimal conditions. The solution is free of unconjugated PerCP and unconjugated antibody.
Concentration	0.2mg/ml
Recommended Usage	Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.
Applications	FC - Quality tested
Application Notes	The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation³, in vitro blocking of B cell responses^1,2, immunohistochemical staining of frozen sections: OCT embedded⁷ and acetone-fixed^4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)), and immunofluorescence microscopy⁹.
Application References	1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block) 2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block) 3. Shen FW, et al. 1986. Immunogenetics 24:146. (IP) 4. Suzuki K, et al. 2000. Immunity 13:691. (IHC) 5. Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC) 6. Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC) 7. Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC) 8. Pascal V, et al. 2007. J. Immunol. 179:1751. (FC) 9. Mende I, et al. 2006. Blood 107:1383. (IF, IHC, FC) 10. Phan TG, et al. 2007. Nature Immunol. 8:992. (FC) 11. Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed 12. Pascal V, et al.2007. J. Immunol. 179:1751. PubMed 13. Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed 14. Takada K, et al. 2009. J. Exp. Med. 206:2253. PubMed 15. Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed 16. Li LX, et al. 2010. J. Immunol. 184:1728. PubMed 17. Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed 18. Kenna TJ, et al. 2008. Blood 111:2091. PubMed 19. Ninjik A, et al. 2012. J Immunol. 189:102. PubMed. "
Description	CD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (e.g., RIII, SJL/J, STS/A, DA). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell types. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1. "
Storage	The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.