FITC anti-human CD95 (Fas)_详细资料

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克隆性：多克隆保存条件：蓝冰规格： 25 tests

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FITC anti-human CD95 (Fas)参数介绍>>>>

Clone DX2
Isotype Mouse IgG1, κ
Reactivity Human, Cross-Reactivity: African Green, Baboon, Capuchin Monkey, Chimpanzee, Common Marmoset, Cotton-topped Tamarin, Cynomolgus, Pigtailed Macaque, Rhesus, Sooty Mangabey
Immunogen CD95 transfected L cells
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
"
Applications
FC - Quality tested
Application Notes
The DX2 antibody is useful for inducing apoptosis of Fas-positive cells. Additional reported applications (for the relevant formats) include: in vitro induction of apoptosis³ (DX2 antibody is required to be cross-linked for effective induction of apoptosis) and immunohistochemical staining^4,5 of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections. The LEAF™ Purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 305614).

Note: EOS9.1 antibody (cat. No. 305703/305704) can induce apoptosis without cross-linking process.
Application References
1. Schlossman S, et al. Eds.1995. Leucocyte Typing V. Oxford University Press. New York.
2. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. New York.
3. Cifone M, et al. 1994. J. Exp. Med. 180:1547. (Apop)
4. Zietz C, et al. 2001. Am. J. Pathol. 159:963. (IHC)
5. Sergi C, et al. 2000. Am. J. Pathol. 156:1589. (IHC)
6. Xie S, et al. 2010. J. Immunol. 184:2289. (FC) PubMed
7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
8. Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21.
9. Rout N, et al. 2010. PLoS One 5:e9787. (FC)
"
Description
CD95 is a 45 kD single chain type I glycoprotein also known as Fas, APO-1, and TNFRSF6. It is a member of the TNF receptor superfamily. CD95 is expressed on T and B lymphocytes, monocytes, neutrophils, and fibroblasts. CD95 expression is upregulated by activation. The extracellular region of CD95 binds to CD178 (Fas ligand). CD178 binding to CD95 induces apoptosis and has been shown to play a role in the maintenance of peripheral tolerance.
Storage The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

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FITC anti-human CD95 (Fas)

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Clone	DX2
Isotype	Mouse IgG1, κ
Reactivity	Human, Cross-Reactivity: African Green, Baboon, Capuchin Monkey, Chimpanzee, Common Marmoset, Cotton-topped Tamarin, Cynomolgus, Pigtailed Macaque, Rhesus, Sooty Mangabey
Immunogen	CD95 transfected L cells
Formulation	Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation	The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Recommended Usage	Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change. "
Applications	FC - Quality tested
Application Notes	The DX2 antibody is useful for inducing apoptosis of Fas-positive cells. Additional reported applications (for the relevant formats) include: in vitro induction of apoptosis³ (DX2 antibody is required to be cross-linked for effective induction of apoptosis) and immunohistochemical staining^4,5 of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections. The LEAF™ Purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 305614). Note: EOS9.1 antibody (cat. No. 305703/305704) can induce apoptosis without cross-linking process.
Application References	1. Schlossman S, et al. Eds.1995. Leucocyte Typing V. Oxford University Press. New York. 2. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. New York. 3. Cifone M, et al. 1994. J. Exp. Med. 180:1547. (Apop) 4. Zietz C, et al. 2001. Am. J. Pathol. 159:963. (IHC) 5. Sergi C, et al. 2000. Am. J. Pathol. 156:1589. (IHC) 6. Xie S, et al. 2010. J. Immunol. 184:2289. (FC) PubMed 7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) 8. Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21. 9. Rout N, et al. 2010. PLoS One 5:e9787. (FC) "
Description	CD95 is a 45 kD single chain type I glycoprotein also known as Fas, APO-1, and TNFRSF6. It is a member of the TNF receptor superfamily. CD95 is expressed on T and B lymphocytes, monocytes, neutrophils, and fibroblasts. CD95 expression is upregulated by activation. The extracellular region of CD95 binds to CD178 (Fas ligand). CD178 binding to CD95 induces apoptosis and has been shown to play a role in the maintenance of peripheral tolerance.
Storage	The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.