Ultra-LEAF™ Purified anti-mouse CD28_详细资料

产品信息

克隆性：多克隆保存条件：蓝冰规格： 1 mg

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Ultra-LEAF™ Purified anti-mouse CD28参数介绍>>>>

Clone 37.51
Isotype Syrian Hamster IgG
Reactivity Mouse
Immunogen C57BL/6 mouse T-cell lymphoma EL-4
Formulation 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration 1mg/ml
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Applications
FC - Quality tested
IP, IHC, Costim, Block - Reported in the literature
Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation¹, in vitro costimulation of T and NK cells¹, in vitro blocking of allogeneic mixed leukocyte response and inhibition of MHC-unrestricted CTL cytotoxicity^3,4, in vitro induction of thymocyte differentiation^2,5-9,11, and immunohistochemical staining of acetone-fixed frozen sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 102112). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 102116) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.
Application References
1. Gross JA, et al. 1992. J. Immunol. 149:380. (IP, Costim)
2. Cibotti R, et al. 1997. Immunity 6:245. (Costim)
3. Masten BJ, et al. 1997. Am. J. Respir. Cell Mol. Biol. 16:335. (Block)
4. Nishio M, et al. 1996. J. Immunol. 157:4347. (Block)
5. Zhang N and He Y-W, 2005. J. Exp. Med. 202:395. (Costim)
6. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Costim)
7. Perchonock CE, et al. 2006. Mol Cell Biol. 26(16):6005. (Costim)
8. Wang W, et al. 2007. J. Immunol. 178:4885. (Costim)
9. Pua HH, et al. 2007. J. Exp. Med. 204:25. (Costim)
10. Perchonock CE, et al. 2007. J. Immunol. 179:1768.
11. Barbi J, et al. 2007. Blood 110:2215.
12. Milpied P, et al. 2011. Blood 118:2993. PubMed
13. Cunningham NR, et al. 2011. Int Immunol. 23:693. PubMed

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Description
CD28 is a 44 kD glycoprotein, also known as Tp44 or T44. It is a member of the Ig superfamily, expressed on thymocytes, most peripheral T cells, and NK cells. In association with CD80 (B7-1) and CD86 (B7-2), CD28 acts as the second signal for T and NK cell activation and proliferation. The 37.51 antibody has been reported to augment in vitro T cell proliferation and cytokine production, and promote CTL development.
Storage The antibody solution should be stored undiluted at 4°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.

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Clone	37.51
Isotype	Syrian Hamster IgG
Reactivity	Mouse
Immunogen	C57BL/6 mouse T-cell lymphoma EL-4
Formulation	0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation	The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration	1mg/ml
Recommended Usage	Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Applications	FC - Quality tested IP, IHC, Costim, Block - Reported in the literature
Application Notes	Additional reported applications (for the relevant formats) include: immunoprecipitation¹, in vitro costimulation of T and NK cells¹, in vitro blocking of allogeneic mixed leukocyte response and inhibition of MHC-unrestricted CTL cytotoxicity^3,4, in vitro induction of thymocyte differentiation^2,5-9,11, and immunohistochemical staining of acetone-fixed frozen sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 102112). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 102116) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg). This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.
Application References	1. Gross JA, et al. 1992. J. Immunol. 149:380. (IP, Costim) 2. Cibotti R, et al. 1997. Immunity 6:245. (Costim) 3. Masten BJ, et al. 1997. Am. J. Respir. Cell Mol. Biol. 16:335. (Block) 4. Nishio M, et al. 1996. J. Immunol. 157:4347. (Block) 5. Zhang N and He Y-W, 2005. J. Exp. Med. 202:395. (Costim) 6. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Costim) 7. Perchonock CE, et al. 2006. Mol Cell Biol. 26(16):6005. (Costim) 8. Wang W, et al. 2007. J. Immunol. 178:4885. (Costim) 9. Pua HH, et al. 2007. J. Exp. Med. 204:25. (Costim) 10. Perchonock CE, et al. 2007. J. Immunol. 179:1768. 11. Barbi J, et al. 2007. Blood 110:2215. 12. Milpied P, et al. 2011. Blood 118:2993. PubMed 13. Cunningham NR, et al. 2011. Int Immunol. 23:693. PubMed "
Description	CD28 is a 44 kD glycoprotein, also known as Tp44 or T44. It is a member of the Ig superfamily, expressed on thymocytes, most peripheral T cells, and NK cells. In association with CD80 (B7-1) and CD86 (B7-2), CD28 acts as the second signal for T and NK cell activation and proliferation. The 37.51 antibody has been reported to augment in vitro T cell proliferation and cytokine production, and promote CTL development.
Storage	The antibody solution should be stored undiluted at 4°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.