产品名称: 总蛋白测试盒 | QuantiChrom™ Protein Assay Kit
分类名称 : 代谢产物分析
产品介绍 : 测定方法 Bradford比色法(595nm)定量测定总蛋白的浓度。
产品说明 蛋白质被称为“生命的物质基础”,它是生命科学领域最重要的大分子之一。蛋白质由多肽氨基酸组成,它在生物体内发挥着各种关键的作用。蛋白质定量检测是生 命科学家常用的方法。简单、直接、自动化的蛋白质浓度检测方法是最为理想的。本公司生产的蛋白质测试法是基于改进的考马斯蓝G的方法。显色剂与蛋白质经过 复杂反应形成蓝色,其颜色强度(595nm)与样品中的蛋白质浓度成正比。优化配方大幅减少了原样品中物质的干扰以及对多肽的更为敏感。
产品用途 直接检测样品蛋白质的总含量。
产品特点 灵敏、准确:用10 mL样品。测试范围 0.06 – 1.0 mg/mL 蛋白质.简单、高通量: 测试过程仅需添加工作试剂及读取吸光度。易于自动化高通量测试,每天可检测几千份样品。低干扰:葡萄糖、维生素、氨基酸、DNA、 RNA、盐、 EDTA (< 12 mM) 、 苯酚 (< 50 mM) 、 尿素 (< 0.6 M) 、表面活性剂 (< 0.1%) 和SDS (< 0.1% SDS)均不影响测试。多功能性: 测试可用96孔板或比色皿进行。
储藏条件:室温运输 。试剂和标准品分别在室温和 -20°C 下保存。
Description The protein is known as the "building blocks of life" and is one of the most important macromolecules in life science. Proteins are polypeptides made up of amino acids and play various key roles in all aspects of biology. Protein quantitation is a very common practice for life scientists. Simple, direct and automation-ready procedures for measuring protein concentration are very desirable. our QuantiChrom
TM protein assay kit is based on an improved Coomassie Blue G method. The dye forms a blue complex specifically with protein, and the intensity of color, measured at 595nm, is directly proportional to the protein concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples and exhibits increased sensitivity towards peptides.
Applications Direct Assays
: total protein concentration.
Key features Sensitive and accurate. Use 10 mL samples. Detection range 0.06 – 1.0 mg /mL protein in 96-well plate assay.Simple and high-throughput. The “mix-and-read” procedure involves addition of a single working reagent and reading the optical density. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.Low interference. Glucose, Tris, vitamins, and amino acids, DNA, RNA, salts, EDTA (< 12 mM), phenol (< 50 mM), urea (< 0.6 M), Triton (< 0.1%) and SDS (< 0.1% SDS) do not interfere in the assay.Versatility: assays can be executed in 96-well plate or cuvet.
Storage conditions. The kit is shipped at room temperature. Store the reagent at room temperature and standard at -20°C, respectively. Shelf life of at least 6 months (see expiry dates on labels).
其他 : 编号:QCPR-048,QCPR-500
测试:48T,500T
公司简介: 博世生物提供优质、快速(如2分钟)、方便的生化检测试剂盒,广泛适用于人和动物等生物样品、食品和环境监测样品中相关指标的检测。博世专注于无害、无放 射性的检测技术(如吸收光、荧光、生物化学发光法等),安全可靠,适合各类仪器(分光光度仪、微孔读数仪等)。产品具有快速、简单、高通量(可用于HTS 药物筛选)、灵敏(特别适合小鼠样品) 、准确性高和低干扰等主要特征。检测步骤简便,用户不需花额外的时间来作调适测试。
博世生物产品主要包括:1、血液尿液化学;2、酶活性测定;3、能量代谢;4、氧化应激;5、高通量HTS试剂;6、阴阳离子检测。
温馨提示:不可用于临床ZL。