预筛，人微血管内皮细胞，新生儿，Pre-Screened Human Dermal Microvascular Endothelial Cells: CADMEC/HMVEC, neonatal

Cat.#S100K-05n
预筛，人微血管内皮细胞，新生儿，Pre-Screened Human Dermal Microvascular Endothelial Cells(CADMEC/HMVEC), neonatal。CADMEC，Pre-Screened来源于正常成人皮肤毛细血管，第2代时冻存于含有10% FBS 和 10% DMSO 的细胞基础培养基，细胞量>5×10⁵cells/ml ，可以达到16倍增。每个批次经检测均不含有HIV、HBV、HCV、支原体、细菌和真菌。

试剂盒包含：预筛人微血管内皮细胞，新生儿(S100-05n，1ml)；人微血管内皮细胞培养基(112-500，500ml) ；细胞外基质包被的T-25培养瓶（121-25-02，2个）；附着因子溶液 (123-100，100ml)；传代培养试剂盒 (090K，100ml)。

推荐培养基：112-500、112K-500

运输条件： 干冰运输、 液氮保存

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VEGF-Stimulated Signaling in Pre-Screened Endothelial Cells
VEGF receptor-2 (VEGFR-2) is a major VEGF of endothelial cells.  VEGFR-2-mediated signaling plays a critical role in angiogenesis, including regulation of proliferation, differentiation, cell movement, and survival of endothelial cells.  VEGF-induced receptor dimerization triggers activation of VEGFR-2 tyrosine kinase and autophosphorylation at a specific set of tyrosine residues, which serve as docking sites for downstream signaling components leading to activation of downstream signaling molecules, including phosphorylation of Akt and p44/42-MAPK.

Expression of Signaling Biomarkers in Pre-Screened Endothelial Cells
Prescreened Endothelial Cells from Cell Applications, Inc. express the following important biomarkers:

• Tie2, a receptor tyrosine kinase critical for the angiogenic remodeling, sprout formation, survival of endothelial cells and vessel stabilization processes;
• eNOS (endothelial Nitric Oxide Synthase), the enzyme that producse NO which is an important signaling molecule that regulates a diverse range of physiological events and is required for normal endothelial function;
• Axl, a receptor tyrosine kinase whose ligand is the survival factor Gas6 (growth arrest-specific gene 6 product) implicated in many processes, such as cell survival, leukocyte transmigration and neointima formation.
• Etk/Bmx (Endothelial/epithelial Tyrosine Kinase), a member of the Btk family, participates in signal transduction stimulated by growth factor receptors, cytokine receptors, G-protein-coupled receptors, antigen receptors, and integrins, and has been implicated in cell adhesion, migration, proliferation, and survival.