ABI 4472908染料法实时荧光定量试剂盒 SYBR® Select Master Mix, 1-Pack (1 x 5 mL) 品名 | 货号 | 规格 |
SYBR® Select Master Mix, 1-Pack (1 x 5 mL) | 4472908 | 500 reactions |
描述
SYBR® Select Master Mix is a superior and cost-effective solution for your real-time PCR applications.
• Specific—minimize primer-dimer and non-specific amplification
• Reproducible and sensitive—consistent amplification across a wide dynamic range
• Bright—contains SYBR® GreenER™ dye for maximum brightness
• Carry-over contamination control—contains heat-labile UDG
• Broad instrument compatibility
Formulated for Maximum Specificity
SYBR® Select Master Mix contains all the components needed for your real-time PCR reaction, except the template and primers, in a convenient 2X concentration premix. The master mix includes AmpliTaq® DNA Polymerase, UP, a highly purified DNA polymerase with a proprietary hot start mechanism that provides exceptional specificity.
Obtain Reproducible Results Across a Wide Dynamic Range
SYBR® Select Master Mix is specially formulated to provide robust results from 100 ng to 0.1 pg cDNA per reaction and can reliably detect a single copy of genomic DNA.
Contains SYBR® GreenER™ Dye and Heat-labile UDG
• SYBR® GreenER™ dye is less inhibitory to PCR than SYBR® Green I dye resulting in brighter signals
• Heat-labile uracil-DNA glycosylase (UDG) is included for worry-free carryover contamination control
Instrument Compatibility
SYBR® Select Master Mix is used in standard cycling mode and is compatible with all Applied Biosystems® real-time PCR instruments. It is also compatible with the Bio-Rad IQ™5, Roche LightCycler® LC480, and Stratagene® MX3005P® systems.
For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
内容和储存
The 2x mix contains SYBR® GreenER™ dye, AmpliTaq® DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye and optimized buffer components. Contains 1 X 5 mL tube, sufficient for 500, 20 µL reactions (10 µL Master Mix per reaction) . Store at 2 - 8 °C.
详细说明
常用规格
Detection Method: | SYBR |
Polymerase: | AmpliTaq® DNA Polymerase |
Purity or Quality Grade: | UP (Ultra Pure) |
Platform: | StepOne™, Standard Mode, ViiA™ 7 System, 7900HT System, Standard Mode, StepOnePlus™, Standard Mode, 7500 System, QuantStudio™ 12K Flex |
Reaction Speed: | Standard |
Sample Type (General): | DNA |
Sample Type (Specific): | cDNA, Genomic DNA |
Primer-Probe Compatibility: | Unlabeled Oligos |
Form: | Liquid |
Format: | Vial(s) |
Hot Start: | Built-In Hot Start |
PCR Method: | qPCR |
Label or Dye: | SYBR® GreenER™ |
Product Size: | 500 reactions |
cGMP Compliance: | non-cGMP Compliant |
Shipping Condition: | Wet Ice |
Regulatory Statement: | For Research Use Only. Not for use in diagnostic procedures. |
Passive Reference Dye: | ROX (Pre-mixed) |
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