Renin Fluorogenic Substrate

Renin is an aspartyl protease of approximately 40 kDa that converts angiotensinogen into angiotensin I.{12331} The renin fluorogenic substrate consists of the normal peptide substrate for renin which has been linked to the fluorophore EDANS at one end and to a non-fluorescent quenching molecule (Dabcyl) at the other.{12969} After cleavage by renin, the product (peptide-EDANS) is brightly fluorescent and can be easily analyzed using an excitation wavelength of 340 nm and emission wavelengths of 485-510 nm.