A frequent cause of high background staining is due to non-specific binding of secondary antibodies. High quality secondary antibodies are crucial to obtaining good staining of targets that are normally harder to detect, for example due to low expression levels. Interference caused by endogenous immunoglobulins can also result in poor detection. Secondary antibodies specifically adsorbed against cross-reacting immunoglobulins will enhance results. ALX-211-502 is recommended for use with select ALEXIS recombinant fusion proteins containing the Fc portion of human IgG.
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