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Yeast Lytic Enzyme
0836095 YEAST LYTIC ENZYME 5000 UNITS
From Arthrobacter luteus
Lytic Activity: 5000 units/g
This enzyme catalyzes the lysis of yeast cells and hydrolyzes glucose polymers with β-1,3-linkages.

Form: Free-flowing, off-white powder.
Source: Arthrobacter species.
References:
1. Doi, K., Doi, A., Fukui, T., J. Biochem., 70, 711 (1971).
2. Doi. K., Doi, A., Fukui, T., Agr. Biol. Chem., 37, 1619 (1973).
3. Doi, K., Doi, A., Ozaki, T., Agr. Biol. Chem., 37, 1629 (1973).


Zymolyase-100T From Arthrobacter luteus

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Description: Zymolyase-100T is an enzyme preparation from a submerged culture of Arthrobacter luteus1 which effectively lyses cell walls of viable yeast cell2,3. This preparation is lyophilized enzyme partially purified by affinity chromatography and contains 100,000 units enzyme/g. The primary enzyme in this preparation responsible for lysis of viable yeast cells in ?-1,3-glucan laminaripentaohydrolase. It hydrolyzes glucose polymers at the ?-1,3-glucan linkages releasing laminaripentaose as the principal product.

Unit Definition: The amount of activity causing a decrease in absorbance at 800 nm of 30%.
Specificity: The lytic spectrum of this enzyme includes organisms from the following genera4: Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloekera, Kluyveromyces, Lipomyces, Metschikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomcopsis, Saccharomycodes, Schwanniomyces and others.

Use: May be used for cell lysis, spheroplasting and glucan hydrolysis.
General: The extent of yeast cell lysis will vary with yeast strain, growth stage and cultural conditions 5-7.

Note: Zymolyase is a registered trademark of the Kirin Brewery Co., Ltd.
Storage: Store at 2 - 8°C in the lyophilized state. Approximately 90% of activity is lost when stored at 30°C for 3 months.
Appearance: Lyophilized powder
Activity: 100,000 units/g
Essential Enzyme: ?-1,3-glucan laminaripentaohydrolase.
Other Enzymes3:

• ?-1,3-glucanase, ca. 1.0 x 107 units/g.
  Protease, ca. 1.7 x 104 units/g.
  Mannase, ca. 6.0 x 104 units/g.
  DNase, RNase (none detectable).

Activators: Dithiothreitol, 2-mercaptoethanol and other sulfhydryl compounds (such as cystein).
Optimum pH and temperature:
pH 7.5, 35° for lysis of viable yeast cells.
pH 6.5, 45° for hydrolysis of yeast glucan.

pH Range: Stable through pH range 5 - 10
Heat stability: Lytic activity is lost in 5 minutes at 60°.
Precautions: Zymolase?-100T has a low solubility. Use as a suspension. If it is necessary to make a sterile enzyme solution of more than 0.05%, make a 2% enzyme stock solution by dissolving Zymolase? -100T in a buffer solution (pH 7.5) which contains 5% glucose. Pipette suspension leaving any material that has sedimented in the container. Nitrocellulose filters are not recommended. Dilute sterile enzyme suspension on to desired concentration.

Kitalase, from Rhizoctonia solami/ 细胞裂解酶，来自茄属丝核菌 1g