Dihydrorhodamine 123,二氢罗丹明123,109244-58-8

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试剂描述：

产品名称：Dihydrorhodamine 123

产品别名：DHR 123; 2-(3,6-Diamino-9H-xanthene-9-yl)-benzoic acid methyl ester

CAS号：109244-58-8

英文别名：DHR 123; 2-(3,6-Diamino-9H-xanthene-9-yl)-benzoic acid methyl ester

分子量：346.38

分子式：C₂₁H₁₈N₂O₃

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保存方法：Store at -20° C

描述：

Dihydrorhodamine 123 (DHR 123) is a cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) such as peroxide and peroxynitrite. It is not fluorescent until oxidized by ROS to the highly fluorescent product Rhodamine 123 . Neither NO, superoxide, nor hydrogen peroxide alone appear to oxidize DHR, rather, these ROS are thought to combine with other cellular components such as cytochrome c oxidase or Fe²⁺ in order to oxidize DHR 123 to its fluorescent derivative Rhodamine 123. Formation of Rhodamine 123 can be monitored by fluorescence spectroscopy using excitation and emission wavelengths of 500 and 536 nm, respectively, or by absorbance spectroscopy at 500 nm (ε = 78,800 M^-1cm^-1).

应用：A fluorescent probe commonly used for determination of peroxynitrite & other ROSs

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