Anti-Cyclooxygenase 2/COX2前列腺素过氧化物合成酶2抗体

本公司提供科研Cyclooxygenase 2/COX2前列腺素过氧化物合成酶2抗体，抗体质量可靠，订购Cyclooxygenase 2/COX2前列腺素过氧化物合成酶2抗体请联系在线客服或者销售人员。
抗体参数如下>>>>
中文名称：前列腺素过氧化物合成酶2抗体
英文名称：Anti-Cyclooxygenase 2/COX2
货号：bs-0732R
抗体来源：兔
克隆类型：多克隆
蛋白分子量：predicted molecular weight: 66kDa
纯化方法：affinity purified by Protein A
交叉反应：rat, mo, hu, Rb, dog, pig, shp, cow
测试应用：ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
产品背景介绍：COX2 (Cyclooxygenase 2) is an inducible enzyme. It is involved in the response of cells to growth factors, tumor promoters, and cytokines that induce its expression. Given its role in synthesizing prostaglandins, COX2 is therefore of interest in studying immune response regulation. COX2 is induced by a wide variety of stimuli and was initially identified as immediate early growth response gene. In addition, COX2 expression markedly increased in 85-90% of human colorectal adenocarcinoma whereas COX1 levels remain unchanged.Function : Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.Subcellular Location : Microsome membrane. Endoplasmic reticulum membrane.Similarity : Belongs to the prostaglandin G/H synthase family.Contains 1 EGF-like domain.合成与降解（Synthesis and Degradation） COX-2 前列腺素氧化环化酶-2是前列腺素合成的关键酶之一，为诱导性酶，他参与细胞对生长因子、肿瘤促进因子和细胞因子的反应，这些因子均能诱导COX2表达，它也参与前列腺素的合成。 COX2在80-90％的人结肠腺癌中表达显著ZG，而COX1表达却不变。产品图片Tissue/cell: rat tongue tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Cyclooxygenase 2/COX2 Polyclonal Antibody, Unconjugated(bs-0732R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingTissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Cyclooxygenase 2/COX2 Polyclonal Antibody, Unconjugated(bs-0732R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining