鸟苷酸酶检测试剂盒/GTPase assay system_详细资料

产品信息

宿主： NA 供应商：上海高创抗原来源： NA 适应物种： NA 是否单克隆：多克隆抗体英文名： GTPase assay system 规格： 5 x 480 assays

Introduction
Colorimetric assays for GTPases are invariably based on the formation of colored complexes between an inorganic phosphate and a dye molecule under acidic conditions.

Competitor assays are beset with several problems including reagent precipitation. At the heart of Innova Biosciences assay kit is PiColorLock™ Gold a non-radioactive, superior phosphate detection reagent which ensures high stability of the colored dye-phosphate complexes (green color- see below).

PicolorLock™ Gold has also been designed to:

Prevent background signals arising from non-enzymatic (i.e. acid) hydrolysis of the substrate. The figure below shows ATP incubated in three detection reagents. A steadily rising background signal is seen with competitor reagents, whereas PiColorLock™ gives baseline readings.

Have a large linear range, thus reducing the need for sample dilution.

Together with specially purified substrates and proprietary stabilizers Innova Biosciences kits offer the lowest possible assay background and outstanding assay performance. Uniquely, Innova Biosciences GTPase assay kits contain highly purified GTP, which have had any free inorganic phosphate removed – this means the assays have the lowest possible background signal.
PiColorLock™ Gold can also be purchased for developing simple assays for any phosphate-generating enzyme.

References:

GTPase Assay Kit

Defective Axonal Transport of Rab7 GTPase Results in Dysregulated Trophic Signaling - The Journal of Neuroscience (2013)

Phosphorylation and nitration of tyrosine residues affect functional properties of Synaptophysin and Dynamin I, two proteins involved in exo-endocytosis of synaptic vesicles - BBA Molecular Cell Research (2012)

The selenocysteine-specific elongation factor contains a novel and multi-functional domain - The Journal of Biological Chemistry (2012)

Tubulin nucleotide status controls Sas-4-dependent pericentriolar material recruitment - Nature Cell Biology (2012)

The Mitochondrial Phosphatase PGAM5 Functions at the Convergence Point of Multiple Necrotic Death Pathways - Cell (2012)

Leucyl-tRNA Synthetase Is an Intracellular Leucine Sensor for the mTORC1-Signaling Pathway - Cell (2012)

ArfGAP1 Is a GTPase Activating Protein for LRRK2: Reciprocal Regulation of ArfGAP1 by LRRK2 - Journal of Neuroscience (2012)

GTPase Activity and Neuronal Toxicity of Parkinson's Disease–Associated LRRK2 Is Regulated by ArfGAP1 - PLoS Genetics (2012)

AIP1 Functions as Arf6-GAP to Negatively Regulate TLR4 Signaling - JBC (2010)

GTPase activity plays a key role in the pathobiology of LRRK2 - PLoS Genetics (2010)

Role of the G-protein and tyrosine kinase—Rho/ROK pathways in 15-hydroxyeicosatetraenoic acid induced pulmonary vasoconstriction in hypoxic rats - J Biochem (2010)

AIP1 Functions as Arf6-GAP to Negatively Regulate TLR4 Signaling - JBC (2010)

Identification and Characterization of a Leucine-Rich Repeat Kinase 2 (LRRK2) Consensus Phosphorylation Motif - PLoS One (2010)

Speckled-like Pattern in the Germinal Center (SLIP-GC), a Nuclear GTPase Expressed in Activation-induced Deaminase-expressing Lymphomas and Germinal Center B Cells - JBC (2009)

High-level production and characterization of a G-protein coupled receptor signaling complex - FEBS Journal (2009)

Structure of ERA in complex with the 3′ end of 16S rRNA: Implications for ribosome biogenesis - PNAS (2009)

G Protein-Coupled Receptor-Promoted Trafficking of Gβ1γ2 Leads to AKT Activation at Endosomes via a Mechanism Mediated by Gβ1γ2-Rab11a Interaction – Molecular Biology of the Cell (2008)

Characterization of Human GTPBP3, a GTP-Binding Protein Involved in Mitochondrial tRNA Modification - Molecular and Cellular Biology (2008)

Comparative real-time PCR and enzyme analysis of selected gender-associated molecules in Schistosoma japonicum - Parasitology (2008)

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