高通量比色法三磷酸腺苷酶检测试剂（High Throughput Colorimetric ATPase Assay Kit）

高通量比色法三磷酸腺苷酶检测试剂（High Throughput Colorimetric ATPase Assay Kit）
 

This non radioactive colorimetric assay kit contains all the necessary reagents for measuring enzyme activity (everything included in the PiColorlock™ reagent and more) and is ideal for high throughput drug screening.

The kits contain PiColorlock™, a non-radioactive, superior phosphate detection reagent.

Key features of PiColorLock™ include:

• Colorimetric Assay – competitor assays are radioactive
• Special additive ensures low backgrounds with acid-labile substrates
• Unparalleled stability of phosphate-dye complexes
• Reagent is compatible with almost any assay buffer
• No inhibition of color development by high concentrations of protein
• Stable reagent formulation – long shelf life

Colorimetric assays for ATPases are invariably based on the formation of colored complexes between an inorganic phosphate and a dye molecule under acidic conditions.

Comparison with competitors:

Competitor assays are beset with several problems including reagent precipitation. The high stability of PiColorLock™ ensures high stability of the colored dye-phosphate complexes (green color- see below).