现货,Hesperadin，目录号S1529,CAS号:422513-13-1，Aurora KinaseYZ剂，美国Selleck_详细资料

产品信息

英文名： Hesperadin CAS号： 422513-13-1 保质期：两年供应商：美国Selleck南京办事处保存条件：零下二十摄氏度低温保存数量：大量规格： 5mg

信号转导通路: HDAC-HSP-Aurora >> Aurora Kinase >> Aurora Kinase YZ剂 >> Hesperadin
http://selleck.cn/hesperadin-S1529.html

技术数据:
分子量(MW): 516.65
化学式:
C29H32N4O3S

溶解度: DMSO ≥103mg/mL Water <1mg/mL Ethanol ≥3mg/mL
纯度: >99%
稳定性: at -20℃ 2 years
CAS号: 422513-13-1

生物活性
Hesperadin is a human Aurora B inhibitor with an IC50 of 40 nM for the prevention of the phosphorylation of substrate. Growth of cultured bloodstream forms (BF) was also sensitive to Hesperadin with an IC50 of 50 nM. It blocked nuclear division and cytokinesis, but not other aspects of the cell cycle. Mammalian cells treated with Hesperadin enter anaphase in the presence of numerous monooriented chromosomes, many of which may have both sister kinetochores attached to one spindle pole (syntelic attachment). Hesperadin also causes cells arrested by taxol or monastrol to enter anaphase within <1 h, whereas cells in nocodazole stay arrested for 3-5 h. [1][2][3]

参考文献
The cell cycle as a therapeutic target against Trypanosoma brucei: Hesperadin inhibits Aurora kinase-1 and blocks mitotic progression in bloodstream forms Neal Jetton, Karen G. Rothberg,et al. Mol Microbiol 2009 April;72(2): 442–458
The small molecule Hesperadin reveals a role for Aurora B in correcting kinetochore–microtubule attachment and in maintaining the spindle assembly checkpoint Silke Hauf,Richard W. Cole,et al. The Journal of Cell Biology 2003;161：281–294
Mechanism of Aurora B Activation by INCENP and Inhibition by Hesperadin Fabio Sessa,Marina Mapelli,et al. Molecular Cell April 29, 2005;18：379–391

客户反馈数据

Western blot analysis of p-histone and histone. 0-10μM Hesperadin was added.

HeLa cells treated with nocodazole containing DMSO or Hesperadin (100 nM) for 2h and stained with the indicated antibodies.

Time-lapse analysis of duration of mitotic arrest in U2OScells treated with Hec1 siRNA and indicated inhibitors in cells non-induced or induced to express LAP–Mis12–Mps1Δ200. Data indicate cumulative percentage of cells (from 50 cells per treatment) that exit mitosis (scored as cell flattening) at the indicated times after NEB, and are representative of three independent experiments.

如果需要长期保存，请于零下二十度低温保存。
禁止用于人体及ZL！

特定的存储和包装每个产品的信息在产品说明书上都有注明。大多数Selleck产品，在推荐的条件下存储可稳定保存两年。产品有时建议的储存温度不同，大多数建议储存在-20 ° C ，抗体及蛋白等产品建议-60℃。YZ剂属于化学试剂，可在常温下运输储存两周左右。即使如此，我们保证产品的出货量将保持产品质量的条件下，一般都会放入冰袋。望阁下收到产品后，请按照产品数据表建议适当存储。

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温馨提示：不可用于临床ZL。

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