现货,SB 203580，目录号S1076,CAS号:152121-47-6，p38 MAPKYZ剂，美国Selleck

信号转导通路:  MAPK >> p38 MAPK >> p38 MAPK YZ剂 >> SB 203580
http://selleck.cn/sb-203580-S1076.html

技术数据:
分子量(MW): 377.43 
化学式: 
C21H16FN3OS

溶解度: DMSO ≥76mg/mL   Water <1mg/mL   Ethanol ≥5mg/mL
纯度: >99%
稳定性: at -20℃ 2 years
CAS号: 152121-47-6

生物活性
SB 203580 is a potent inhibitor of LPS-induced cytokine synthesis in the human monocyte cell line THP-1 (IC50 = 50-100 nM). [1] SB 203580 inhibited the RK from heat-shocked HeLa cells and osmotically stressed PC12 cells or bacterially expressed CSBP-2 with an IC50 of 0.6 μM.
SB 203580-induced activation of c-Raf is independent of stress-activated protein kinase 2a/p38 inhibition. [2]

参考文献
SB 203580 is a specific inhibitor of a MAP kinase homologue which is stimulated by cellular stresses and interleukin-1 Ana Cuenda,John Rouse,et al. FEBS Letters 1995;364：229－233
Use of a drug-resistant mutant of stress-activated protein kinase 2a/p38 to validate the in vivo specificity of SB 203580 Patrick A. Eyersa,et al. FEBS Letters. 1999;191-196.

客户反馈数据
 
A, effects of p38 inhibitor SB203580 (1, 5, and 10 M) on SRP72 protein expression was evaluated by WB using antibodies against human SRP72,SRP54, and GAPDH. A decreased intensity of SRP72 bands was noted when using the inhibitor at 5M concentration at 240 min (lane 6) and 10M at 120 and 240 min (lanes 8 and 9). B, results were analyzed and RUA illustrated, finding significant results at 5M, 240 versus 0 min, and 10M, 240 versus 0 and 120 versus 0 min, respectively, (p0.05). 

 A, effect of p38 MAPK inhibitor SB203580 (1, 5, and 10 M) on SRP72 phosphorylation was evaluated. Jurkat cells were stimulated with rhIL-1, harvested at 0, 120, and 240 min, lysed, and immunoprecipitated using anti-human SRP19 andWBusing anti-phosphoserine antibodies. A decreased intensity of SRP72 band was found when using the inhibitor at concentration of 1M at 240 min and 5 and 10M at 120 and 240 min. B, results were analyzed and RUA illustrated, finding significant results at 1M 240 versus 120, 5, and 10M at 120 versus 0 and 240 versus 0 min *, p0.05.


 We used real time RT-PCR to investigate the effects of SB203580 on SRP72 mRNA expression. All the samples were treated under the same experimental conditions used in protein tests done by WB, and phosphorylation was measured indirectly by IP-WB. The results did not show significant changes on the mRNA expression of SRP72 after the treatment with these inhibitors.

如果需要长期保存，请于零下二十度低温保存。
禁止用于人体及ZL！

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