美国进口，现货，ABT-737 ，目录号 S1002，CAS号:852808-04-9，细胞凋亡(Apoptosis) ，Bcl-2 YZ剂，美国Selleck_详细资料

产品信息

数量：大量保存条件：零下二十摄氏度保存英文名： ABT-737 CAS号： 852808-04-9 供应商：美国Selleck南京办事处保质期：两年规格： 5mg

信号转导通路:  细胞凋亡(Apoptosis) >> Bcl-2 >> Bcl-2 YZ剂 >> ABT-737
http://www.selleck.cn/abt-737-S1002.html
技术数据:

分子量(MW): 813.43

化学式:
C₄₂H₄₅ClN₆O₅S₂

溶解度: DMSO ≥163mg/mL   Water <1mg/mL   Ethanol <1mg/mL

纯度: >99%

稳定性: at -20℃ 2 years

CAS号: 852808-04-9

生物活性

ABT-737 is a pan-Bcl-2 inhibitor that has a wide range of single-agent activity against acute lymphoblastic leukemia (ALL) cell lines and xenografts. Increased expression of the Bcl-2 family of proteins in cancers has been associated with chemotherapy resistance,^[1-3]inhibiting Bcl-2 or Bcl-XL overexpression could potentially induce apoptosis in cancer cells while having minimal effects on normal cells. The ABT-737 single-agent LC90 values ranged from 100 nM for COG-LL-319 and RS4;11 cells to low micromolar concentrations for the other five ALL cell lines.

参考文献

Prognostic Role of bcl-xL and p53 in Childhood Acute Lymphoblastic Leukemia (ALL) Raffaele Addeo,Michele Caraglia,et al. Cancer Biology & Therapy January 2005;4：32-38

Clinical relevance of BCL-2 overexpression in childhood acute lymphoblastic leukemia E Coustan-Smith, A Kitanaka,et al. Blood 1996;87: 1140-1146

客户反馈数据

Effect of ABT-737 on the cell viability of CCRF-CEM cells by treatments of AY4 (10 lg/ml), TRAIL (0.5 lg/ml), SAHA (1 lM),VPA (1 mM), or ABT-737 (10 lM) alone or in combination for 24 h prior to MTT assay.

Upper panel ABT-737 inhibits TFK-1 and EGI-1 cell growth.Cells were exposed to ABT-737 at a concentration ranging from 1 to 50 lM. Following 72 h of incubation, growth inhibition was analyzed by crystal violet assay. Dose–effect plot of ABT-737 treatment is presented.

Lower panel detection of PARP-1, cleaved caspase-9 and caspase-3, BCL-2 and MCL-1 in TFK-1 and EGI-1 cells after 72 h of ABT-737 treatment (1, 3, 10, 25,50 lM). Cell lysates were analyzed on Western blotting.

3 mM ABT737 inhibited growth and viability of TF-1 cells and potentiated proapoptotic effects of 1 mM BIO after 72 hours treatment. TF-1 cells treated with both drugs exhibited more apoptotic cells compared to those treated with each single drug. ABT737 abrogated the protection from BIO-induced apoptosis provided by MS5 coculture.

BCL-XL mediates human neutrophil survival. PMNs were preincubated with the BH3 mimetic ABT-737 (1–10 μM), then cultured in normoxia (gray bars) with or without GM-CSF (500 U/ml) or hypoxia (white bars)or 20 hours, and apoptosis was assessed by morphology (n = 4).

Bcl-XL/Bcl-2 inhibitor ABT-737 aggravates the proapoptotic effects of IL-1IFN-. INS-1E cells were transfected with single or smart Pool PUMA siRNAs and exposed to ABT-737 for 24 h. At this time point, cell death was measured by HO/PI, n 3. *, p 0.05; **, p 0.01.

MEF wt and MEF Mcl-1 ko mice activating active caspase-3 using 1um ABT for 24h

MDB-MA-231 cells were exposed to 30 um cisplatin in the absence or in thepresence of 100nm ABT-737.The cell were stained with Hoechst 33342,MitoTracker Red and Yo-pro-1.

如果需要长期保存，请于零下二十度低温保存。

禁止用于人体及ZL！

特定的存储和包装每个产品的信息在产品说明书上都有注明。大多数Selleck产品，在推荐的条件下存储可稳定保存两年。产品有时建议的储存温度不同，大多数建议储存在-20 ° C ，抗体及蛋白等产品建议-60℃。YZ剂属于化学试剂，可在常温下运输储存两周左右。即使如此，我们保证产品的出货量将保持产品质量的条件下，一般都会放入冰袋。望阁下收到产品后，请按照产品数据表建议适当存储。

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温馨提示：不可用于临床ZL。

分子量(MW):	813.43
化学式:	C₄₂H₄₅ClN₆O₅S₂
溶解度:	DMSO ≥163mg/mL Water <1mg/mL Ethanol <1mg/mL
纯度:	>99%
稳定性:	at -20℃ 2 years
CAS号:	852808-04-9

	Effect of ABT-737 on the cell viability of CCRF-CEM cells by treatments of AY4 (10 lg/ml), TRAIL (0.5 lg/ml), SAHA (1 lM),VPA (1 mM), or ABT-737 (10 lM) alone or in combination for 24 h prior to MTT assay.
	Upper panel ABT-737 inhibits TFK-1 and EGI-1 cell growth.Cells were exposed to ABT-737 at a concentration ranging from 1 to 50 lM. Following 72 h of incubation, growth inhibition was analyzed by crystal violet assay. Dose–effect plot of ABT-737 treatment is presented.
	Lower panel detection of PARP-1, cleaved caspase-9 and caspase-3, BCL-2 and MCL-1 in TFK-1 and EGI-1 cells after 72 h of ABT-737 treatment (1, 3, 10, 25,50 lM). Cell lysates were analyzed on Western blotting.
	3 mM ABT737 inhibited growth and viability of TF-1 cells and potentiated proapoptotic effects of 1 mM BIO after 72 hours treatment. TF-1 cells treated with both drugs exhibited more apoptotic cells compared to those treated with each single drug. ABT737 abrogated the protection from BIO-induced apoptosis provided by MS5 coculture.
	BCL-XL mediates human neutrophil survival. PMNs were preincubated with the BH3 mimetic ABT-737 (1–10 μM), then cultured in normoxia (gray bars) with or without GM-CSF (500 U/ml) or hypoxia (white bars)or 20 hours, and apoptosis was assessed by morphology (n = 4).
	Bcl-XL/Bcl-2 inhibitor ABT-737 aggravates the proapoptotic effects of IL-1IFN-. INS-1E cells were transfected with single or smart Pool PUMA siRNAs and exposed to ABT-737 for 24 h. At this time point, cell death was measured by HO/PI, n 3. , p 0.05; *, p 0.01.
	MEF wt and MEF Mcl-1 ko mice activating active caspase-3 using 1um ABT for 24h
	MDB-MA-231 cells were exposed to 30 um cisplatin in the absence or in thepresence of 100nm ABT-737.The cell were stained with Hoechst 33342,MitoTracker Red and Yo-pro-1.

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美国进口，现货，ABT-737 ，目录号 S1002，CAS号:852808-04-9，细胞凋亡(Apoptosis) ，Bcl-2 YZ剂，美国Selleck

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美国进口，现货，ABT-737 ，目录号 S1002，CAS号:852808-04-9，细胞凋亡(Apoptosis) ，Bcl-2 YZ剂，美国Selleck