冷冻切片质量提升系统CryoJane™ Tape-Transfer System
CryoJane™ Tape-Transfer System,可切取 2 micros 超薄冷冻切片,提升切片品质,避免破片、组织变形,可以解决fatty tissue & undecal cified bone 的切片问题,适用于 LCM 使用者。
整个系统含以下内容物(见图示):
1. Flash-Pad Mechanism
2. Electronics Control Unit
3. Power Cord
4. Door Channel
5. Storage Shelf
6. Hand Roller
7. 125 Adhesive- Coated Slides
8. Roll of 400 Tape Windows
9. 2 Knife Facet Wipers
10. Spare Fuse and 5 Block Holders (optional)
产品选购:
货号 | 产品名称 | 规格 | 价格 |
62800-20 | CryoJane™ Tape-Transfer System | 220Volt | 询价 |
使用步骤如下:
1. 将TAPE WINDOWS (组织贴片带) 贴在蜡块表面
2. 用锋利的切片刀切出适当厚度的frozen section
3. 将整片粘有组织的 TAPE WINDOWS (组织贴片) 移到Adhensive coated slides
4. 利用hand roller 将组织贴片更加平贴在slides上
5. 将slides 移到Flash-Pad (UV 灯箱)中照射数秒
6. UV会将slides上的coating转换成可固定住组织的solvent-resistant plastic
7. 移除(组织贴片带)
8. 可以继续后续的fixation & staing步骤
CryoJane™小附件简介:
货号 | 品名 | 产品描述 | 规格 | 图片 |
62800-40 | Block-Holder | Deepwaffled, large face blockholder securely anchors frozen embedding medium or tissue. | 5/pk | |
62800-44 | Microtome Chuck Adapter | Adapts to the microtome chuck for the use of BlockHolders above. (Please specify cryostat model when ordering.) | 1 |
62800-46 | Hand Roller | Required in the CryoJane™ process for laminating Tape Window to block-face | 1 | |
62800-48 | Storage Shelf | Used for storing Adhesive Coated Slides and Tape Window inside the cryostat. | 1 | |
62800-50 | Door Channel | Please specify model cryostat and whether Cryo-Vac-Away is installed. | 1 | |
62800-53 | Fuses-1-Amp | SLO-BLOW ceramic core wound. Used in the CryoJane™ ECU | 5/pk |
62800-X | Adhesive Slides | Glass slide with adhesive coating, when polymerized, bonds the still frozen section from the tape to the slide. Avoid thawing the section. ½ x coating slides for mild to normal tissues. | 125/pk | |
62800-1X | Adhesive Slides | Glass slide with adhesive coating, when polymerized, bonds the still-frozen section from the tape to the slide. Avoid thawing the section. 1x coating slides for mild to normal tissues. | 125/pk | |
62800-4X | Adhesive Slides | Glass slide with adhesive coating, when polymerized, bonds the still-frozen section from the tape to the slide. Avoid thawing the section. 4x coating slides for use with hard un-calcified bone) or fatty tissues. | 100/pk | |
62800-72 | Tape Windows | Applied to the block-face with the Hand Roller to capture the frozen section as it is being cut. Eliminate the need for a brush or anti-roll device. | 400/pk | |
62800-74 | Protective Oil | Used for coating frozen section blocks to prevent dehydration during long-term storage. | 4OZ | |
62803-30 | Protective Oil Accessories Kit | Temperature controlled Oil Bath, 4 oz Bottle of Protective Oil, and Oil Brush. Please note that installation of the Protective Oil Bath Accessory requires that the CryoJane™ system be returned to Electron Microscopy Sciences. | 1 |
62800-76 | Aqueous Buffer Salt Mix | Used in the preparation of the aqueous fixative, which preserves morphological detail in frozen sections. 2 vials per package, (each vial makes 180 ml of buffer). | 2/pk | |
62800-78 | Knife Facet Wipers | Effectively cleans both facets of knife-edge without dulling the blade. 10 wipers/pack | 10/pk |