上海普飞生物技术有限公司：021-51088100 http://www.pufei.com/
3D细胞培养趋势:
3D细胞培养为细胞更好的生长、分化及功能提供更适合的环境：
Ÿ 在Z少的外源支撑和干扰条件下，维持单个细胞正常的三维形态和结构，
Ÿ 支持细胞与相邻的细胞间保持复杂的相互联系，及传输各种信号，
Ÿ 创造更接近于自然组织结构的环境进行培养，
Ÿ 减少细胞在扁平的2D培养表面上针对各种人为的压力做出的响应。
细胞生长环境改变对细胞行为的影响：
| | 传统的2D细胞培养 | Alvetex 3D细胞培养 | 正常体内环境 |
| 正常尺寸和生理差异 |
| 细胞获取营养的距离 | 0 | 0-100μm | 0-200μm |
| 与体内细胞环境类似性 | 低 | 高 | n/a |
| 细胞形态 | 扁平 | 立体 | 立体 |
| 三维细胞相互联系可能性 | 非常低 | 高 | 高 |
| 形成复杂三维细胞结构能力 | 非常低 | 高 | 高 |
| Z初的塑料制品上接种细胞 |
| 细胞压力对于细胞结构的影响 | 高 | 低 | n/a |
| 蛋白与基因表达变化 | 高 | 低 | n/a |
| 细胞与塑料接触表面面积 | >50% | 0-50% | n/a |
| 接种后 |
| 细胞形状不断变化 | 高 | 低 | n/a |
| 是否需要重塑细胞支架构建 | 高 | 低 | n/a |
| 与正常体内形态的偏移度 | 高 | 低 | n/a |
| 细胞与塑料制品接触面积 | >50% | 0-50% | n/a |
| 增强体外细胞功能性的机会 | 低 | 高 | n/a |
什么是Alvetex®？
Alvetex®是一种高度疏松的交联聚苯乙烯支架，形成的疏松孔洞直径约36-40μm，孔洞间的连通缝隙约12-14μm，并被截成200μm厚的薄片，该材料是化学惰性的，并且已经做成了适合常规细胞培养板的形式。
Alvetex®提供了一个非常合适的3D结构，细胞可以在这样更合适的环境里进行增殖、迁移、分化及执行各种生物学功能。细胞保持3D形态，并与相邻的细胞建立更紧密的相互联系。
Alvetex特点：
| Alvetex特点 | 用alvetex培养3D细胞培养的好处 |
| 与现常用的细胞培养塑料制品相同材料 | 轻松切换模式，无需对培养试剂做出任何调整 |
| 惰性 — 细胞生长与功能无影响，不会产生新的实验误差 |
| 稳定 — 不会降解，长时间研究无变化，可以预铺ECM蛋白 |
| 一致性的支架结构—极少的批间差异（ISO认证） | 可重复性好，一致的结果，批间差异小 |
| 真正的均一的3D细胞生长 |
| 完整支架只有200um厚 | 细胞与营养和气体距离不会大于100μm，模拟体内环境 |
| 细胞可以通过主动扩散吸收和排泄 — 模拟体内环境 |
| >90%的成孔率 | 细胞可以轻松的穿入支架，释放基质 — 更加贴近体内环境 |
| 细胞和培养基可轻松穿过支架 |
| 营养与废弃物质通过被动扩散进行 |
| 部分细胞修复成为可能 |
| 孔洞直径为36-40μm | 大约75个细胞能占满一个孔（根据细胞种类而有所不同） |
订购信息:
| 货 号 | 产 品 说 明 |
| AVP002 | 12孔培养板形式，Alvetex圆片已经固定在孔底 |
| AVP004-3 | 细胞小室形式，Alvetex圆片固定在小室底部， 配适6孔培养板（需自备），3个独立包装 |
| AVP005-3 | 细胞小室形式，Alvetex圆片固定在小室底部， 配适6/12孔培养板（需自备），3个独立包装 |
| AVP015 | 带有三联支架的培养深皿，可支持任意3个细胞小室形式的Alvetex，（本身不带有Alvetex） |
所有产品都经过伽马照射灭菌并保持无菌包装，所有Alvetex圆片都可轻松卸取。
如果您对真正的3D细胞培养技术有兴趣，请联系您身边的普飞销售人员，或在网站的“客户留言”留下您的单位及联系方式，我们会及时与您联系！
alvetex®3D细胞培养主页：http://www.reinnervate.com/
alvetex® Frequently Asked Questions
Q: Are there any published papers concerning the use of alvetex®?
A: There are numerous peer-reviewed scientific articles available describing the development and application of alvetex®.
Q: Can I coat alvetex® with known proteins and reagents?
A: Yes. Most standard coating methods for cell culture plastic are compatible with alvetex®. For example, Collagen I and IV, Fibronectin, Laminin, Basement Membrane Extract have all been tested. See technical brochure showing example of collagen coated scaffold.
Q: Can I extract protein and nucleic acid from cells grown in alvetex®?
A: Yes. Cells can be disrupted and lysed within the scaffold using standard reagents for subsequent collection of total protein and nucleic acid.
Q: Can I remove alvetex® from the culture plate/insert?
A: Yes. alvetex® can easily be removed from the culture plate or well insert.
Q: Can I remove my cells from the scaffold?
A: Yes. Cells can be removed from the scaffold using a combination of trypsin and mild agitation. About 60-70% of cells can be removed in this way.
Q: Can I see cells using standard light microscopy?
A: alvetex® has been developed to enable 3D cell growth which it achieves very successfully. The visualisation of cells in any 3D system will always be challenging. More sophisticated methods such as confocal microscopy can be used to see cells cultured on alvetex® (see technical brochure). Depending on the cell density, it is possible to pick out individual cells growing within large voids.
Q: Can I use more than one scaffold within the same well?
A: It is possible to place more than one scaffold in a well or use the custom 6-well insert designed to house multiple scaffolds. This technology allows users for the first time the ability to study the interaction of two cultured cell types grown in 3D in an easy to handle system. Users can grow primary cells, or cells representative of a particular disease, and study their interaction with normal cells in these 3D models. This technology is designed to be very versatile with the user in mind.
Q: Does alvetex® come ready to use?
A: Like standard cell culture plastic-ware, alvetex® is ready to use from the moment it is unwrapped.
Q: Does alvetex® degrade?
A: alvetex® is inert and does not degrade during standard cell culture practice.
Q: Does alvetex® enable 3D cell growth?
A: Cells grown in alvetex® adopt a 3D geometry. Cells grow in contact with adjacent cells forming a thin 3D layer.
Q: Does the material auto fluoresce?
A: alvetex® does not auto fluoresce under the standard conditions we have tested for fluorescent microscopy.
Q: Has alvetex® been treated in any way?
A: alvetex® has been surface activated using the standard methods commonly used in preparing polystyrene for cell culture. alvetex® has also been sterilised using standard techniques.
Q: How do I seed cells onto alvetex®?
A: There are various ways of doing this. For example, users can seed cells in a small volume of medium (e.g. 200µl) centrall onto the scaffold, leave for 15min, and then carefully add the remaining culture medium. Alternatively, a cell suspension can be made using the full amount of culture medium and added directly to alvetex®. We recommend in the first instance, the user optimise the system for their purposes.
Q: How is alvetex® supplied?
A: alvetex® will be supplied packaged either in multi-well culture plates or in individual blister packed well inserts designed to fit all standard 6, 12 and 24 well plates.
Q: How much culture media do I use?
A: For any given culture vessel type, cultures grown in 3D contain more cells than their 2D counterparts. Accordingly, the metabolic demands on that media are generally higher in 3D. In a standard plate, the vessel should be filled with media to its maximum recommended level. To address this issue, a series of new devices has been developed to enable users to culture cells in 3D using alvetex® with larger volumes of culture media.
Q: How often do I need to change the culture medium?
A: This depends on the nature of the cells grown in 3D culture. Within a standard culture vessel, a 3D culture will require more frequent media changes compared to its 2D counterpart. However, this requires optimisation
for the cell type concerned. A series of new devices has been developed to enable users to culture cells in 3D using alvetex® with larger volumes of culture media. This equipment allows fewer media changes and maintains culture media quality.
Q: Is alvetex® available in a well insert?
A: alvetex® will eventually be available in well inserts as 6, 12 and 24-well formats.
Q: Is alvetex® compatible with other technologies?
A: alvetex® is a versatile material that can adapted for use in a broad variety of applications. We encourage investigators to explore its use in alternative and novel areas. Please enquire should you require advice about your particular application.
Q: Is alvetex® easy to use?
A: alvetex® is very straightforward to use. It has been developed specifically for routine 3D cell cultureand can be used alongside existing cell culture products. Full instructions are provided.
Q: Is alvetex® re-useable?
A: No, like any plastic consumable product for cell culture, it is not recommended to re-use alvetex®.
alvetex®3D细胞培养主页：http://www.reinnervate.com/
上海普飞生物技术有限公司：021-51088100 http://www.pufei.com/
