己糖激酶/己糖磷酸激酶/HK/Hexokinase from Saccharomyces cerevisiae

英文名称：HK；Hexokinase from Saccharomyces cerevisiae；ATP:D-Hexose-6-phosphotransferase；Hexokinase from yeast
其他名称：己糖磷酸激酶
CAS号：9001-51-8

级别：BR

类型：Type F-300
分子量：~ 54 kDa (monomer)；~110 kDa (dimer)
活力：≥130 units/mg protein (biuret)

活力定义：One unit will phosphorylate 1.0 μmole of D-glucose per min at pH 7.6 at 25℃
Optimal pH：7.5～9.0

Extinction Coefficient：E1% = 8.85 (PI) and 9.47 (PII) at 280 nm

Anion traces：sulfate (SO42-)：free

Foreign activity：ATPase, myokinase, glucose-6-phosphate dehydrogenase, 6-phosphogluconic dehydrogenase and phosphoglucose isomerase ≤0.01%
性状：白色鳞状粉末。酿酒酵母提取。催化剂(Activators)：Hexokinase requires Mg2+ ions (KM = 2.6 mM) for activity. Hexokinase is activated by catecholamines and related compounds；YZ剂(Inhibitors)：sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxy-methylglucose, xylose, lyxose, and thiol reactive compounds (Hg2+ and 4-chloromercuribenzoate)
用途：生化研究。为催化己糖6位上由ATP使之产生磷酸化反应的酶。对α－D葡萄糖的亲和性很高(Km=1×10－5M)，对其他的己糖也可进行磷酸化。此反应的平衡向右偏移。△G°’=4.0千卡。该酶可被Mg2 、 K 所活化，但可被HS试剂阻抑。
保存：-20℃