T4多聚核苷酸激酶,T4 Polynucleotide Kinase

T4 Polynucleotide Kinase, 3′-phosphatase freefrom phage T4 am N81 pse T1 infected Escherichia coli BB

别名: kinase, t4 polynucleotide, 3′-phosphatase-free

产品描述
Application

Use T4 Polynucleotide Kinase, 3′-phosphatase-free, to:
• Phosphorylate the 5′ ends of RNA or DNA
• Phosphorylate the 3′ ends of RNA to prevent cyclization
• Add a 5′[32P]-terminal label to 3′-CMP, forming 5′[32P]pCp. This substrate is commonly used for 3′-end labeling of RNA with T4 RNA ligase.
• Label 5′-hydroxyl ends of DNA with [?-32P]-ATP (either by direct phosphorylation or by phosphate exchange)
• Phosphorylate the 5′-hydroxyl ends of oligonucleotides obtained from automated synthesizers
• Phosphorylate the 5′-hydroxyl ends of linkers.
• Purify recombinant RNA in E. coli.

Note: The 3′-phosphatase activity of the wild-type kinase is not observed under optimal incubation conditions with the mutant T4 Polynucleotide Kinase.

T4 polynucleotide kinase catalyzes the transfer of the terminal phosphate group of ATP to the 5′-hydroxyl terminus of DNA or RNA. It also can catalyze the exchange of 5′-terminal phosphate groups. In contrast to the wild type E. coli enzyme, this phage derivative lacks 3′-phosphatase activity. The assay time is 30 minutes and volume activity is 10000 U/ml.

包装

1 kit containing 2 components

Roche试剂

10709557001	T4 Polynucleotide Kinase, 200 ,T4多聚核苷酸激酶
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