-Anti-phospho-Ser/Thr-Pro, MPM-2, agarose conjugate

Description:

Anti-phospho-Ser/Thr-Pro, MPM-2, agarose conjugate

 

Specificity:

Recognizes a phosphorylated epitope (LTPLK or YWFSPL)6,7 in a distinct class of phosphoproteins including MAP2, HSP70, cdc25, and DNA topoisomerase IIα, most of which are phosphorylated at the onset of mitosis. The number of phosphoproteins recognized by MPM2 varies from species to species and with the type of cell.

 

Key Applications:

• Immunocytochemistry
• Immunoprecipitation

 

Application Notes:

Suitable for immunoprecipitation and affinity purification.

 

Species Reactivity:

All

 

Species Reactivity Note:

Both animals and plants.

 

Immunogen:

Mitotic human HeLa cell cytosolic lysate.

 

Modifications:

Phosphorylation

 

Presentation:

1 mg of MPM2 antibody covalently coupled to 1 mL of protein G agarose beads and provided as a 50% slurry suspended in PBS with 0.05% sodium azide for a final volume of 2 mL.

 

Storage Conditions:

1 year at 4°C

 

Quality Assurance:

routinely evaluated by immunoprecipitating mitotic MPM2 antigens from colcemid treated human HeLa cells as shown by subsequent immunoblot using the monoclonal anti-phospho-Ser/Thr-Pro, MPM2 (05-368)

 

Antibody Category:

Epigenetics & Nuclear Function

 

Antibody Sub-Category:

Cell Cycle, DNA Replication & Repair

 

Usage Statement:

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

 

Trade Name:

Upstate (Millipore)

 

Format:

Gel Immobilized

 

Host:

Mouse

 

Product Overview:

This antibody recognizes a variety of proteins that are phosphorylated during mitosis.

MPM2 (Mitotic Protein Monoclonal #2, 05-368) cross-linked to protein G agarose by dimethylpimelimidate

 

Antibody Type:

Monoclonal Antibody