l 锋利、无菌 单独包装其他需要准备的物品
1. 小型弯头解剖剪刀curved scissors
2. 纱布Gauze pads
3. 组织镊forceps
4. ***伏、消毒酒精
5. 创可贴
6. 消毒纸巾
实验步骤举例:
Raise an intradermal wheal with anesthetic agent. Stabilize the skin with the
thumb and forefinger, stretching it slightly perpendicular to the normal skin
tension lines; this will produce an oval rather than round defect, facilitating
closure.
Place the punch perpendicular to the skin and apply firm and constant
downward pressure with a circular twisting motion. Removal of the punch to
"check the progress" should be avoided as this may result in a ragged wound and
a shredded biopsy sample. A definite "give" occurs when the punch reaches the
subcutaneous fat, indicating that a full-thickness cut has been made. Remove the
punch and apply downward finger pressure at the sides of the wound to pop up
the core. Completely elevate the core with gentle use of forceps or a needle tip
and excise it at its base with small tissue scissors. The pressure applied through
forceps to elevate the core should be gentle to minimize the destruction and
distortion of tissue in the biopsy sample. After finishing the harvest of the tissue,
prepare the biopsy site for closure。