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MYCOLOGY COLLECTION
ATCC® NUMBER: 10231™
ORGANISM: Candida albicans
Strain (designation) ID: NIH 3147
Genotype: Not Available.
CITATION OF STRAIN:
If use of this strain results in a scientific publication it
should be cited in that manuscript in the following
manner: Candida albicans ATCC® 10231™.
PRODUCT DEscriptION:
An ampoule containing viable cells (may include spores
and mycelia) suspended in cryoprotectant.
BIOSAFETY LEVEL: 1
Appropriate safety procedures should always be used
with this material. Laboratory safety is discussed in the
following publication: Biosafety in Microbiological and
Biomedical Laboratories, 5th ed. from the U.S.
Department of Health and Human Services Centers for
Disease Control and Prevention and National Institutes
for Health. The entire text is available online at
www.cdc.gov/od/ohs/biosfty/bmbl5/bmbl5toc.htm.
PROPAGATION:
The information recommended in this section is to assist
users in obtaining living culture(s) for their studies. The
recommendation does not imply that the conditions or
procedures provided below are optimum. Experienced
researchers may initiate the growth of a culture in their
own way. 白色念珠菌 ATCC 10231,念珠菌,ATCC 菌种
Recommended Medium:
Users are encouraged to visit the web page of this
ATCC product at www.atcc.org for additional, updated
product information. The following web site allows for
searching the formulae of an ATCC medium.
http://www.atcc.org/TechnicalSupport/MediaFormulation
s/tabid/760/Default.aspx
ATCC Medium Number:
1245 (YEPD)
200 (Yeast Mold Agar = BD 271210)
28 (Sabouraud Dextrose Agar)
For making a liquid medium, don’t include agar.
Autoclave at 121°C for 15 minutes.
If a supplement is needed, cool the medium to 55°C
(or room temperature for liquid medium) and aseptically
add the supplement, gently mix well; and adjust pH if
necessary.
Recommended Incubation Temperature: 25°C.
Recommended Incubation Atmospheric Conditions:
Typical Aerobic.
Recommended Procedure:
For freeze-dry (lyophilized) ampoules:
1. Open an ampoule according to enclosed
instructions.
2. From a single test tube of sterile distilled water (5
to 6 ml), withdraw approximately 0.5 to 1.0 ml with a
sterile pipette and apply directly to the pellet. Stir to
form a suspension.
3. Aseptically transfer the suspension back into the test
tube of sterile distilled water. 白色念珠菌 ATCC 10231
4. Let the test tube sit at room temperature (25°C)
undisturbed for at least 2 hours; longer (e.g.,
overnight) rehydration might increase viability of
some fungi..
5. Mix the suspension well. Use several drops (or
make dilutions if desired) to inoculate recommended
solid or liquid medium. Include a control that
receives no inoculum.
6. Incubate the inoculum at the propagation conditions
recommended.
7. Inspect for growth of the inoculum/strain regularly.
The sign of viability is noticeable typically after 1-2
days of incubation. However, the time necessary for
significant growth will vary from strain to strain.
Colony and Cell Morphology:
On YEPD agar after 2 days at 25°C, colonies are
cream-colored, shiny, smooth. Older colonies show
filaments-like structure at the margin and may have
ridges or folders. Cells are ovoid (3.0-6.0 x 4.0-8.0
μm), budding, mostly singly and rarely clustered in
young culture. Cells will elongate and form chain-like
branched pseudohyphae in older culture. 白色念珠菌 ATCC 10231
Pantoea stewartii subsp. stewartii ATCC 8199
Clavibacter michiganensis subsp. michiganensis ATCC 492
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