植物总蛋白抽提SD-008/SN-009

Invent Biotechnologies MinuteTM total protein extraction kit for plant tissues is composed of optimized protein extraction buffer and protein extraction filter cartridges with 2.0 ml collection tubes. The kit is designed to rapidly extract denatured or native proteins from plant tissues (leaves, seeds and soft stem and roots etc.). Since protein profiles extracted by denaturing and native cell lysis buffer are not identical, for a given application one might be superior to the other. This kit provides both denaturing and native cell lysis buffers for users to test and select the best one for a specific application. Due to the use of the protein extraction filter cartridges, total plant soluble proteins can be extracted from 20-200 mg plant tissue in less than 8 min with high protein yield (2-8 mg/ml). Extracted proteins can be use as a good starting material for protein analysis and small scale protein purification in column chromatography.

Cat# SD-008/SN-009    

 
Figure 1. Plant protein profiles extracted by denaturing lysis buffer and native lysis buffer. Extracted proteins were separated in 10% SDS-PAGE and stained with coomassie blue. Lane 1, snow pea; lane 2, corn kernel; 3, broccoli leaves; 4, spinach leaves and 5, chili pepper leaves.

 

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