QIAGEN RNeasy PowerSoil Total RNA Kit (25) 土壤RNA提取MOBIO 12866-25

 

• Optimized lysis for tough samples – Isolate total RNA from all soil types, includign difficult environmental samples
• Inhibitor Removal Technology - Eliminates humic substances and other PCR inhibitors, for RNA that is ready to use in RT-qPCR and RNA-seq
• High RNA yield - Purifies RNA from up to 2 grams of soil for increased yield even from low biomass samples

The RNeasy PowerSoil Total RNA Kit utilizes our novel and proprietary Inhibitor Removal Technology for isolating total RNA from up to 2 grams of soil. RT-PCR and PCR inhibiting substances, including humic substances are completely removed. The kit is intended for use with all common soil types, but in particular, with samples that typically contain a high humic substance content, including compost, sediment and manure.

The kit offers an easy-to-follow procedure. Environmental samples are added to a bead beating tube with a kit-supplied buffer for rapid and thorough homogenization. Total RNA is captured on a proprietary matrix in a column format, washed and eluted. RNA is ready to use in RT-qPCR and RNA-seq without further handling or purification. IMPORTANT NOTE: This kit requires the addition of Phenol: Chloroform: Isoamyl Alcohol (not provided).

Try our RNeasy PowerSoil DNA Elution Kit. Now you can co-isolate DNA and RNA from the same starting sample!

Formerly known as the RNA PowerSoil Total RNA Isolation Kit.
 

High RNA Yield and Reliable RT-PCR Results
		Figure 1. Total RNA was isolated from 8 different soil samples using the RNeasy PowerSoil Total RNA Kit (lanes 1-8). RNA was displayed (A) on a 1% x TAE agarose gel (2 µg per lane). RT-PCR analysis with primers representing the Streptomyces spp. (B) and Bacillus spp. (C) was performed using 1 µl of the undiluted RNA and analyzed on a 1% TAE agarose gel and stained with ethidium bromide. N = negative control. P = positive control. Soil types and amounts are identified below. Positive RT-PCR amplification was observed with of the samples.