Cleaved Caspase-3 (Asp175) (1E) Rabbit mAb #9664
active caspase 3 active caspase-3 anti caspase 3 anti caspase-3 Apopain apoptosis casp367 casp39 caspase 3 caspase-3 caspase3 CPP 32 CPP-32 CPP32 SCA-1 Yama Protein
PhosphoSitePlus ® protein, site, and accession data: CASPASE-3
| No. | Size | Price | |
| 9664L | 300 µl ( 30 western blots ) | ￥9,325.00 | |
| 9664S | 100 µl ( 10 western blots ) | ￥3,780.00 | |
| 9664T | 20 µl ( 2 western blots ) | ￥1,300.00 | |
| 9664 | carrier free & custom formulation / quantity | email request |
| Applications | Dilution | Species-Reactivity | Sensitivity | MW (kDa) | Isotype |
| W | 1:1000 | Human,Mouse,Rat,Monkey | Endogenous | 17, 19 | Rabbit IgG |
| IP | 1:50 |
| IHC-P | 1:1000 |
| F | 1:6400 |
| IF-IC | 1:400 |
| IHC-F | 1:800 |
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry), IHC-F=Immunohistochemistry (Frozen)
Homology
Species predicted to react based on sequence homology: Dog
Specificity / Sensitivity
Cleaved Caspase-3 (Asp175) (1) Rabbit mAb detects endogenous levels of the large fragment (17/19 kDa) of activated caspase-3 resulting from cleavage adjacent to Asp175. This antibody does not recognize full length caspase-3 or other cleaved caspases.
Cleaved Caspase-3 (Asp175) (1) Rabbit mAb 兔单抗能够检测活化的邻近Asp175处剪切的caspase-3大片段(17/19 kDa)。该抗体不能识别全长的 caspase-3 和其它caspases的剪切产物。
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues adjacent to Asp175 of human caspase-3.
该单克隆抗体是采用合成的人源caspases-3 Asp175 氨基末端序列相对应的肽段免疫动物后制备获得。
IP
Immunoprecipitation of extracts from Jurkat cells, untreated or etoposide-treated (25uM, 5hrs), using Cleaved Caspase-3 (Asp175) (1E) Rabbit mAb. Western blot was performed using the same antibody.
免疫沉淀分析Jurkat 细胞的提取物，未处理或 etoposide （依托泊苷）(25uM, 5hrs)处理，采用Cleaved Caspase-3 (Asp175) (1E) Rabbit mAb 兔单抗，然后用同样抗体进行western blot 分析。
IHC-P (paraffin)
Immunohistochemical staining of paraffin-embedded mouse embryo, showing cytoplasmic localization in apoptotic cells, using Cleaved Caspase-3 (Asp175) (1E) Rabbit mAb.
免疫组化分析石蜡包埋小鼠胚胎，显示凋亡细胞中裂解的caspase-3定位胞浆，抗体采用 Cleaved Caspase-3 (Asp175) (1E) Rabbit mAb 兔单抗。
IHC-P (paraffin)
Immunohistochemical analysis using Cleaved Caspase-3 (Asp175) (1E) Rabbit mAb on SignalSlide (TM) Cleaved Caspase-3 IHC Controls #8104 (paraffin-embedded Jurkat cell, untreated (left) or etoposide-treated (right)).
免疫组化分析，用 SignalSlide (TM) Cleaved Caspase-3 IHC Controls #8104（石蜡包埋的Jurkat 细胞，未处理（左）或 etoposide 处理（右）），采用 Cleaved Caspase-3 (Asp175) (1E) Rabbit mAb 兔单抗。
Western Blotting
Western blot analysis of extracts from C6 (rat), NIH/3T3 (mouse), and Jurkat (human) cells, untreated or treated with staurosporine (1uM, 3hrs) or etoposide (25uM, 5hrs) as indicated, using Cleaved Caspase-3 (Asp175) (1E) Rabbit mAb.
Western blot 分析未处理或 staurosporine （星胞菌素） (1uM, 3hrs) 或 etoposide （依托泊苷）(25uM, 5hrs)处理的C6 (大鼠源)、NIH/3T3 (小鼠源)和Jurkat (人源) 细胞。抗体采用Cleaved Caspase-3 (Asp175) (1E) Rabbit mAb 兔单抗。
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded mouse embryo, using Cleaved Caspase-3 (Asp175) (1E) Rabbit mAb in the presence of control peptide (left) or Cleaved Caspase-3 (Asp175) Blocking Peptide (#1050) (right).
免疫组化分析石蜡包埋的小鼠胚胎，抗体采用Cleaved Caspase-3 (Asp175) (1E) Rabbit mAb 兔单抗，并用对照肽段（左图）或 Cleaved Caspase-3 (Asp175) 封闭肽段 (#1050) 对照(右图)。
IF-IC
Confocal immunofluorescent images of HT-29 cells, untreated (left) or Staurosporine treated (right) labeled with Cleaved Caspase-3 (Asp175) (1E) Rabbit mAb#9953 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
共聚焦免疫荧光分析未处理（左）或 Staurosporine (星胞菌素)处理（右）的HT-29 细胞，抗体采用 Cleaved Caspase-3 (Asp175) (1E) Rabbit mAb 兔单抗#9953（绿色）。微丝用Alexa Fluor® 555 phalloidin（红色）标记。蓝色伪彩=DRAQ5® #4084 (DNA 荧光染料)。
IHC-F (frozen)
Immunohistochemical analysis of frozen H1650 xenograft, using Cleaved Caspase-3 (Asp175) (1E) Rabbit mAb.
免疫组化分析 H1650 xenograft 冰冻切片，抗体采用Cleaved Caspase-3 (Asp175) (1E) Rabbit mAb 兔单抗。
Background
Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly(ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires aspartic acid at the P1 position (2).
Caspase-3 (CPP-32, Apoptain, Yama, SCA-1)是一个凋亡的关键执行者，因为它对许多关键的蛋白起着部分或全部的裂解作用，比如核酶多聚(ADP-ribose)聚合酶(PARP)（1）。caspase-3的活化需要经过其未激活的的酶原转变成活化的 p17 和 p12 片段的蛋白水解过程。caspase-3的剪切需要天门冬氨酸位于p1位点（2）。
- Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4.
- Nicholson, D.W. et al. (1995) Nature 376, 37-43.
Application References
- Jak, M. et al. (2011) Blood 118, 5178-88.Applications:Western Blotting
- Jane, E.P. et al. (2011) Mol Cancer Ther 10, 198-208.Applications:Western Blotting
- Yerlikaya, A. et al. (2012) Tumour Biol 33, 1385-92.Applications:Western Blotting
- Yoshioka, K. et al. (2012) Nat Med 18, 1560-9.Applications:IF-IC (In Cells),Western Blotting
- Yilmaz, M. et al. (2011) EMBO J 30, 4489-99.Applications:IHC-P (paraffin)
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Protocols
- 9664 :
- Western Blotting Immunoprecipitation (Magnetic) Immunohistochemistry (Paraffin) Flow Immunofluorescence Immunohistochemistry (Frozen)
Companion Products
- 1050 Cleaved Caspase-3 (Asp175) Blocking Peptide
- 12630 SignalFire™ Plus ECL Reagent
- 12757 SignalFire™ Elite ECL Reagent
- 14214 Caspase-3 (D3R6Y) Rabbit mAb (IHC Formulated)
- 14220 Caspase-3 (D3R6Y) Rabbit mAb
- 6883 SignalFire™ ECL Reagent
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7727 Biotinylated Protein Ladder Detection Pack
- 8059 SignalStain® DAB Substrate Kit
- 8104 SignalSlide® Cleaved Caspase-3 (Asp175) IHC Controls
- 8112 SignalStain® Antibody Diluent
- 8114 SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
- 8202 PhosphoPlus® Caspase-3 (Cleaved, Asp175) Antibody Duet
- 9579 Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb
- 9661 Cleaved Caspase-3 (Asp175) Antibody
- 9662 Caspase-3 Antibody
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For Research Use Only. Not For Use In Diagnostic Procedures.
U.S. Patent No. 5,675,063.
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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStain is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
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Cell Signaling CST ，9664S，Cleaved Caspase-3 (Asp175) (1E) Rabbit mAb
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