PowerUp™ SYBR Green qPCR Master Mix_详细资料

产品信息

保存条件： 2-8℃ 供应商：河南艾克斯生物英文名： PowerUp™ SYBR™ Green Master Mix 数量：现货

描述PowerUp SYBR Green 预混液
适用于所有qPCR仪器的通用型预混液
PowerUp^TM SYBR Green 预混液的特性包括：
•双重热启动机制提供了出众的特异性
•在较宽的动态范围内都能获得可重复性的CT值
•适用于标准或快速循环，＜50分钟内即可获得结果
•采用UN G和dUTP配方，可防止残留PCR产物污染
•制备好qPCR反应板侯，可以子啊室温下稳定保存72小时
•与大多数实时qPCR仪器的兼容性
货号A25742

For qPCR users using SYBR green dye who need to minimize optimization time, Applied Biosystems PowerUp SYBR Green Master Mix offers proprietary Dual-Lock hot start technology. Unlike other polymerases, a Dual-Lock polymerase offers two unique hot start mechanisms to prevent primer dimer and non-specific amplification.

PowerUp SYBR Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows. Coupled with user-supplied primer sets and template, PowerUp SYBR Green Master Mix is designed to amplifiy targets for accurate gene expression analysis.

PowerUp SYBR Green Master Mix features include:
• A dual hot-start mechanism for excellent specificity
• Highly reproducible CTs over a broad dynamic range
• Inclusion of UDG to help prevent carryover contamination
• Stability of pre-assembled reactions for up to 72 hours
• Compatibility with most real-time qPCR instruments

Exceptional specificity with dual hot-start mechanism
Specificity is paramount in obtaining high-quality data in SYBR Green reactions and can be enhanced through control of the Taq DNA polymerase at lower temperatures before stringent primer binding. PowerUp SYBR Green Master Mix uses the Dual-Lock Taq DNA polymerase enzyme that combines two unique hot-start mechanisms to help control its activity and to prevent undesirable early activity of the polymerase at low temperatures. In an experiment evaluating 24 different primer sets, PowerUp SYBR Green Master Mix generated single melt curves of the time, consistent with highly specific amplification.

Tight reproducibility in CTs over a wide dynamic range
PowerUp SYBR Green Master Mix demonstrates excellent reproducibility over a wide dynamic range for a variety of targets tested (see figure). In addition, this master mix provides efficient amplification over 6 logs of sample input. Unlike competitor mixes that can exhibit inhibition at high cDNA inputs and low correlation coefficients over a dynamic range experiment, PowerUp SYBR Green Master Mix efficiency is maintained over the full dynamic range to help deliver maximal confidence in data quality (see figure).

UDG included for carryover contamination control¹
The inclusion of uracil-DNA glycosylase (UDG) and dUTP in the PowerUp SYBR Green Master Mix enables the degradation of any previously amplified PCR products, helping prevent contamination of subsequent qPCR reactions and possible false positives.

Stability of pre-assembled reactions
The tight control of the Dual-Lock Taq DNA polymerase in PowerUp SYBR Green Master Mix enables reactions to be assembled up to 72 hours prior to cycling without impacting data (see figure). This consistency allows users to have confidence and flexibility in the use of PowerUP SYBR Green Master Mix across numerous workflow scenarios.²

Broad instrument compatibility
PowerUp SYBR Green Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems real-time PCR instruments.³ It is also compatible with the Bio-Rad IQ™5 and CFX96™/CFX384™ systems, Roche LightCycler™ LC480, and Stratagene™ MX3005P™ systems. For optimal results, recommended primer concentrations are 300–800 nM.
温馨提示：不可用于临床ZL。

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