pFC332真菌基因编辑CRISPR/CAS9 #87845 构巢曲霉

测序及更多信息：  https://pan.baidu.com/s/1CBBDCXK6JU4gaFyW2O7M5Q 密码：s14x

载体作用

AMA1 plasmid with Aspergillus optimized Cas9 and hph(hygR) selection marker

载体骨架

载体抗性

Ampicillin

载体类型

细菌表达， CRISPR，真菌表达

筛选标记

Hygromycin

插入基因信息

Cas9 ，Synthetic; synthetic gene, codon optimized for A. niger

启动子

Aspergillus nidulans（构巢曲霉）ef1 promoter

参考文献

A CRISPR-Cas9 System for Genetic Engineering of Filamentous Fungi. Nodvig CS, Nielsen JB, Kogle ME, Mortensen UH. PLoS One. 2015 Jul 15;10(7):e0133085. doi: 10.1371/journal.pone.0133085. eCollection 2015. PONE-D-15-11561 [pii] PubMed 26177455

载体图谱↑蓝色为测序比对区

全序列

1        ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG
61       TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA
121      GCGCTGCGAT GATACCGCGA GAACCACGCT CACCGGCTCC GGATTTATCA GCAATAAACC
181      AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT
241      CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG
301      TTGTTGCCAT CGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA
361      GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG
421      TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA
481      TGGTTATGGC AGCGCTACAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG
541      TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT
601      CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA
661      TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA
721      GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG
781      TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC
841      GGAAATGTTG AATACTCATA TTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT
901      ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTCA
961      GTGTTACAAC CAATTAACCA ATTCTGAACA TTATCGCGAG CCCATTTATA CCTGAATATG
1021     GCTCATAACA CCCCTTGTTT GCCTGGCGGC AGTAGCGCGG TGGTCCCACC TGACCCCATG
1081     CCGAACTCAG AAGTGAAACG CCGTAGCGCC GATGGTAGTG TGGGGACTCC CCATGCGAGA
1141     GTAGGGAACT GCCAGGCATC AAATAAAACG AAAGGCTCAG TCGAAAGACT GGGCCTTTCG
1201     CCCGGGCTAA TTATGGGGTG TCGCCCTTAT TCGACTCTAT AGTGAAGTTC CTATTCTCTA
1261     GAAAGTATAG GAACTTCTGA AGTGGGGATT TAAATGCGGC CGCGCTGAGG GTTTAATCGA
1321     CGAAGCAGCT GACGGCCAGT GCCAAGCTTA ACGCGTACCG GGCCCAGTAT ATGTTCCGCA
1381     GATGACTGGA GCTCTGCCAT ACGTGCCCTC TCAAGCACCA TTTGTTCCAT CTACAGAGAC
1441     TAGTCACCAA CTAGTCTATC AAGACTCACA GGGTACATTG CTGAGACCAA CTGACCAGAG
1501     GCAGGGTAGC GGATTGACGG CTCCATCTCC TTCACTTACA AGGTCTATTG AAAGCCCTTT
1561     AGCATCACCA AGCGGAGAAT AGATTGTTAA GCTTATTTTT TGTATACTGT TTTGTGATAG
1621     CACGAAGTTT TTCCACGGTA TCTTGTTAAA AATATATATT TGTGGCGGGC TTACCTACAT
1681     CAAATTAATA AGAGACTAAT TATAAACTAA ACACACAAGC AAGCTACTTT AGGGTAAAAG
1741     TTTATAAATG CTTTTGACGT ATAAACGTTG CTTGTATTTA TTATTACAAT TAAAGGTGGA
1801     TAGAAAACCT AGAGACTAGT TAGAAACTAA TCTCAGGTTT GCGTTAAACT AAATCAGAGC
1861     CCGAGAGGTT AACAGAACCT AGAAGGGGAC TAGATATCCG GGTAGGGAAA CAAAAAAAAA
1921     AANCAAGACA GCCACATATT AGGGAGACTA GTTAGAAGCT AGTTCCAGGA CTAGGAAAAT
1981     AAAAGACAAT GATACCACAG TCTAGTTGAC AACTAGATAG ATTCTAGATT GAGGCCAAAG
2041     TCTCTGAGAT CCAGGTTAGT TGCAACTAAT ACTAGTTAGT ATCTAGTCTC CTATAACTCT
2101     GAAGCTAGAA TAACTTACTA CTATTATCCT CACCACTGTT CAGCTGCGCA AACGGAGTGA
2161     TTGCAAGGTG TTCAGAGACT AGTTATTGAC TAGTCAGTGA CTAGCAATAA CTAACAAGGT
2221     ATTAACCTAC CATGTCTGCC ATCACCCTGC ACTTCCTCGG GCTCAGCAGC CTTTTCCTCC
2281     TCATTTTCAT GCTCATTTTC CTTGTTTAAG ACTGTGACTA GTCAAAGACT AGTCCAGAAC
2341     CACAAAGGAG AAATGTCTTA CCACTTTCTT CATTGCTTGT CTCTTTTGCA TTATCCATGT
2401     CTGCAACTAG TTAGAGTCTA GTTAGTGACT AGTCCGACGA GGACTTGCTT GTCTCCGGAT
2461     TGTTGGAGGA ACTCTCCAGG GCCTCAAGAT CCACAACAGA GCCTTCTAGA AGACTGGTCA
2521     ATAACTAGTT GGTCTTTGTC TGAGTCTGAC TTACGAGGTT GCATACTCGC TCCCTTTGCC
2581     TCGTCAATCG ATGAGAAAAA GCGCCAAAAC TCGCAATATG GCTTTGAACC ACACGGTGCT
2641     GAGACTAGTT AGAATCTAGT CCCAAACTAG CTTGGATAGC TTACCTTTGC CCTTTGCGTT
2701     GCGACAGGTC TTGCAGGGTA TGGTTCCTTT CTCACCAGCT GATTTAGCTG CCTTGCTACC
2761     CTCACGGCGG ATCTGCATAA AGAGTGGCTA GAGGTTATAA ATTAGCACTG ATCCTAGGTA
2821     CGGGGCTGAA TGTAACTTGC CYTTCCTTTC TCATCGCGCG GCAAGACAGG CTTGCTCAAA
2881     TTCCTACCAG TCACAGGGGT ATGCACGGCG TACGGACCAC TTGAACTAGT CACAGATTAG
2941     TTAGCAACTA GTCTGCATTG AATGGCTGTA CTTACGGGCC CTCGCCATTG TCCTGATCAT
3001     TTCCAGCTTC ACCCTCGTTG CTGCAAAGTA GTTAGTGACT AGTCAAGGAC TAGTTGAAAT
3061     GGGAGAAGAA ACTCACGAAT TCTCGACACC CTTAGTATTG TGGTCCTTGG ACTTGGTGCT
3121     GCTATATATT AGCTAATACA CTAGTTAGAC TCACAGAAAC TTACGCAGCT CGCTTGCGCT
3181     TCTTGGTAGG AGTCGGGGTT GGGAGAACAG TGCCTTCAAA CAAGCCTTCA TACCATGCTA
3241     CTTGACTAGT CAGGGACTAG TCACCAAGTA ATCTAGATAG GACTTGCCTT TGGCCTCCAT
3301     CAGTTCCTTC ATAGTGGGAG GTCCATTGTG CAATGTAAAC TCCATGCCGT GGGAGTTCTT
3361     RTCCTTCAAG TGCTTGACCA ATATGTTTCT GTTGGCAGAG GGAACCTGTC AACTAGTTAA
3421     TAACTAGTCA GAAACTAGTA TAGCAGTAGA CTCACTGTAC GCTTGAGGCA TCCCTTCACT
3481     CGGCAGTAGA CTTCATATGG ATGGATATCA GGCACGCCAT TGTCGTCCTG TGGACTAGTC
3541     AGTAACTAGG CTTAAAGCTA GTCGGGTCGG CTTACTATCT TGAAATCCGG CAGCGTAAGC
3601     TCCCCGTCCT TAACTGCCTC GAGATAGTGA CAGTACTCTG GGGACTTTCG GAGATCGTTA
3661     TCGCGAATGC TCGGCATACT AATCGTTGAC TAGTCTTGGA CTAGTCCCGA GCAAAAAGGA
3721     TTGGAGGAGG AGGAGGAAGG TGAGAGTGAG ACAAAGAGCG AAATAAGAGC TTCAAAGGCT
3781     ATCTCTAAGC AGTATGAAGG TTAAGTATCT AGTTCTTGAC TAGATTTAAA AGAGATTTCG
3841     ACTAGTTATG TACCTGGAGT TTGGATATAG GAATGTGTTG TGGTAACGAA ATGTAAGGGG
3901     GAGGAAAGAA AAAGTCGGTC AAGAGGTAAC TCTAAGTCGG CCATTCCTTT TTGGGAGGCG
3961     CTAACCATAA ACGGCATGGT CGACTTAGAG TTAGCTCAGG GAATTTAGGG AGTTATCTGC
4021     GACCACCGAG GAACGGCGGA ATGCCAAAGA ATCCCGATGG AGCTCTAGCT GGCGGTTGAC
4081     AACCCCACCT TTTGGCGTTT CTGCGGCGTT GCAGGCGGGA CTGGATACTT CGTAGAACCA
4141     GAAAGGCAAG GCAGAACGCG CTCAGCAAGA GTGTTGGAAG TGATAGCATG ATGTGCCTTG
4201     TTAACTAGGT CAAAATCTGC AGTATGCTTG ATGTTATCCA AAGTGTGAGA GAGGAAGGTC
4261     CAAACATACA CGATTGGGAG AGGGCCTAGG TATAAGAGTT TTTGAGTAGA ACGCATGTGA
4321     GCCCAGCCAT CTCGAGGAGA TTAAACACGG GCCGGCATTT GATGGCTATG TTAGTACCCC
4381     AATGGAAACG GTGAGAGTCC AGTGGTCGCA GATAACTCCC TAAATTCCCT GAGCTAACTC
4441     TAAGTCGACC ATGCCGTTTA TGGTTAGCGC CTCCCAAAAA GGAATGGCCG ACTTAGAGTT
4501     ACCTCTTGAC CGACTTTTTC TTTCCTCCCC CTTACATTTC GTTACCACAA CACATTCCTA
4561     TATCCAAACT CCAGGTACAT AACTAGTCGA AATCTCTTTT AAATCTAGTC AAGAACTAGA
4621     TACTTAACCT TCATACTGCT TAGAGATAGC CTTTGAAGCT CTTATTTCGC TCTTTGTCTC
4681     ACTCTCACCT TCCTCCTCCT CCTCCAATCC TTTTTGCTCG GGACTAGTCC AAGACTAGTC
4741     AACGATTAGT ATGCCGAGCA TTCGCGATAA CGATCTCCGA AAGTCCCCAG AGTACTGTCA
4801     CTATCTCGAG GCAGTTAAGG ACGGGGAGCT TACGCTGCCG GATTTCAAGA TAGTAAGCCG
4861     ACCCGACTAG CTTTAAGCCT AGTTACTGAC TAGTCCACAG GACGACAATG GCGTGCCTGA
4921     TATCCATCCA TATGAAGTCT ACTGCCGAGT GAAGGGATGC CTCAAGCGTA CAGTGAGTCT
4981     ACTGCTATAC TAGTTTCTGA CTAGTTATTA ACTAGTTGAC AGGTTCCCTC TGCCAACAGA
5041     AACATATTGG TCAAGCACTT GAAGGAYAAG AACTCCCACG GCATGGAGTT TACATTGCAC
5101     AATGGACCTC CCACTATGAA GGAACTGATG GAGGCCAAAG GCAAGTCCTA TCTAGATTAC
5161     TTGGTGACTA GTCCCTGACT AGTCAAGTAG CATGGTATGA AGGCTTGTTT GAAGGCACTG
5221     TTCTCCCAAC CCCGACTCCT ACCAAGAAGC GCAAGCGAGC TGCGTAAGTT TCTGTGAGTC
5281     TAACTAGTGT ATTAGCTAAT ATATAGCAGC ACCAAGTCCA AGGACCACAA TACTAAGGGT
5341     GTCGAGAATT CGTGAGTTTC TTCTCCCATT TCAACTAGTC CTTGACTAGT CACTAACTAC
5401     TTTGCAGCAA CGAGGGTGAA GCTGGAAATG ATCAGGACAA TGGCGAGGGC CCGTAAGTAC
5461     AGCCATTCAA TGCAGACTAG TTGCTAACTA ATCTGTGACT AGTTCAAGTG GTCCGTACGC
5521     CGTGCATACC CCTGTGACTG GTAGGAATTT GAGCAAGCCT GTCTTGCCGC GCGATGAGAA
5581     AGGAARGGCA AGTTACATTC AGCCCCGTAC CTAGGATCAG TGCTAATTTA TAACCTCTAG
5641     CCACTCTTTA TGCAGATCCG CCGTGAGGGT AGCAAGGCAG CTAAATCAGC TGGTGAGAAA
5701     GGAACCATAC CCTGCAAGAC CTGTCGCAAC GCAAAGGGCA AAGGTAAGCT ATCCAAGCTA
5761     GTTTGGGACT AGATTCTAAC TAGTCTCAGC ACCGTGTGGT TCAAAGCCAT ATTGCGAGTT
5821     TTGGCGCTTT TTCTCATCGA TTGACGAGGC AAAGGGAGCG AGTATGCAAC CTCGTAAGTC
5881     AGACTCAGAC AAAGACCAAC TAGTTATTGA CCAGTCTTCT AGAAGGCTCT GTTGTGGATC
5941     TTGAGGCCCT GGAGAGTTCC TCCAACAATC CGGAGACAAG CAAGTCCTCG TCGGACTAGT
6001     CACTAACTAG ACTCTAACTA GTTGCAGACA TGGATAATGC AAAAGAGACA AGCAATGAAG
6061     AAAGTGGTAA GACATTTCTC CTTTGTGGTT CTGGACTAGT CTTTGACTAG TCACAGTCTT
6121     AAACAAGGAA AATGAGCATG AAAATGAGGA GGAAAAGGCT GCTGAGCCCG AGGAAGTGCA
6181     GGGTGATGGC AGACATGGTA GGTTAATACC TTGTTAGTTA TTGCTAGTCA CTGACTAGTC
6241     AATAACTAGT CTCTGAACAC CTTGCAATCA CTCCGTTTGC GCAGCTGAAC AGTGGTGAGG
6301     ATAATAGTAG TAAGTTATTC TAGCTTCAGA GTTATAGGAG ACTAGATACT AACTAGTATT
6361     AGTTGCAACT AACCTGGATC TCAGAGACTT TGGCCTCAAT CTAGAATCTA TCTAGTTGTC
6421     AACTAGACTG TGGTATCATT GTCTTTTATT TTCCTAGTCC TGGAACTAGC TTCTAACTAG
6481     TCTCCCTAAT ATGTGGCTGT CTTGTTTTTT TTTTTTGTTT CCCTACCCGG ATATCTAGTC
6541     CCCTTCTAGG TTCTGTTAAC CTCTCGGGCT CTGATTTAGT TTAACGCAAA CCTGAGATTA
6601     GTTTCTAACT AGTCTCTAGG TTTTCTATCC ACCTTTAATT GTAATAATAA ATACAAGCAA
6661     CGTTTATACG TCAAAAGCAT TTATAAACTT TTACCCTAAA GTAGCTTGCT TGTGTGTTTA
6721     GTTTATAATT AGTCTCTTAT TAATTTGATG TAGGTAAGCC CGCCACAAAT ATATATTTTT
6781     AACAAGATAC CGTGGAAAAA CTTCGTGCTA TCACAAAACA GTATACAAAA AATAAGCTTA
6841     ACAATCTATT CTCCGCTTGG TGATGCTAAA GGGCTTTCAA TAGACCTTGT AAGTGAAGGA
6901     GATGGAGCCG TCAATCCGCT ACCCTGCCTC TGGTCAGTTG GTCTCAGCAA TGTACCCTGT
6961     GAGTCTTGAT AGACTAGTTG GTGACTAGTC TCTGTAGATG GAACAAATGG TGCTTGAGAG
7021     GGCACGTATG GCAGAGCTCC AGTCATCTGC GGAACATATA CTGGGCCCGG GAAGATCTCA
7081     TGGTCATAGC TGTTTCCGCT GAGGGTTTAA TTAAGACCTC AGCCGAGACA GCAGAATCAC
7141     CGCCCAAGTT AAGCCTTTGT GCTGATCATG CTCTCGAACG GGCCAAGTTC GGGAAAAGCA
7201     AAGGAGCGTT TAGTGAGGGG CAATTTGACT CACCTCCCAG GCAACAGATG AGGGGGGCAA
7261     AAAGAAAGAA ATTTTCGTGA GTCAATATGG ATTCCGAGCA TCATTTTCTT GCGGTCTATC
7321     TTGCTACGTA TGTTGATCTT GACGCTGTGG ATCAAGCAAC GCCACTCGCT CGCTCCATCG
7381     CAGGCTGGTC GCAGACAAAT TAAAAGGCGG CAAACTCGTA CAGCCGCGGG GTTGTCCGCT
7441     GCAAAGTACA GAGTGATAAA AGCCGCCATG CGACCATCAA CGCGTTGATG CCCAGCTTTT
7501     TCGATCCGAG AATCCACCGT AGAGGCGATA GCAAGTAAAG AAAAGCTAAA CAAAAAAAAA
7561     TTTCTGCCCC TAAGCCATGA AAACGAGATG GGGTGGAGCA GAACCAAGGA AAGAGTCGCG
7621     CTGGGCTGCC GTTCCGGAAG GTGTTGTAAA GGCTCGACGC CCAAGGTGGG AGTCTAGGAG
7681     AAGAATTTGC ATCGGGAGTG GGGCGGGTTA CCCCTCCATA TCCAATGACA GATATCTACC
7741     AGCCAAGGGT TTGAGCCCGC CCGCTTAGTC GTCGTCCTCG CTTGCCCCTC CATAAAAGGA
7801     TTTCCCCTCC CCCTCCCACA AAATTTTCTT TCCCTTCCTC TCCTTGTCCG CTTCAGTACG
7861     TATATCTTCC CTTCCCTCGC TTCTCTCCTC CATCCTTCTT TCATCCATCT CCTGCTAACT
7921     TCTCTGCTCA GCACCTCTAC GCATTACTAG CCGTAGTATC TGAGCACTTC TCCCTTTTAT
7981     ATTCCACAAA ACATAACACA ACCTTCACCA TGGACAAGAA GTATAGCATC GGGCTGGACA
8041     TTGGAACGAA CTCGGTTGGT TGGGCTGTGA TTACGGACGA ATACAAGGTG CCATCCAAGA
8101     AGTTTAAGGT CCTGGGAAAC ACCGACCGTC ACTCAATCAA GAAGAATCTC ATTGGAGCCC
8161     TGCTCTTCGA TAGTGGGGAG ACCGCCGAAG CTACTCGACT GAAGCGAACG GCTCGCCGGC
8221     GTTATACACG ACGCAAGAAT CGCATCTGCT ACCTCCAGGA GATTTTCAGC AACGAAATGG
8281     CTAAGGTTGA TGACTCATTC TTTCATCGAC TCGAAGAAAG TTTCTTGGTC GAGGAGGATA
8341     AGAAGCACGA GCGCCATCCG ATCTTTGGTA ACATTGTGGA TGAGGTTGCC TATCACGAAA
8401     AGTACCCAAC TATCTATCAT CTTCGTAAGA AGCTGGTCGA TAGCACGGAC AAGGCTGATT
8461     TGCGACTTAT CTACCTGGCA CTCGCGCACA TGATTAAGTT CCGCGGCCAT TTTCTTATCG
8521     AGGGTGACCT GAACCCCGAT AATTCTGACG TTGATAAGCT CTTCATCCAG TTGGTCCAAA
8581     CCTACAATCA GCTGTTTGAG GAAAACCCTA TTAATGCATC TGGCGTGGAC GCCAAGGCTA
8641     TCCTTTCGGC GCGCCTGTCT AAGTCGCGGC GTTTGGAGAA CCTTATCGCA CAACTCCCCG
8701     GCGAAAAGAA GAACGGCCTC TTCGGTAATT TGATTGCGTT GTCACTTGGT CTGACTCCTA
8761     ACTTCAAGAG TAATTTTGAC CTGGCAGAGG ATGCGAAGCT CCAGTTGTCT AAGGATACGT
8821     ATGATGACGA TCTCGACAAC TTGCTTGCCC AAATCGGTGA CCAGTACGCT GATCTTTTCC
8881     TGGCCGCTAA GAATCTCTCA GATGCAATCC TGCTCAGTGA CATTTTGCGG GTCAACACCG
8941     AGATTACTAA GGCCCCCCTG TCAGCTAGTA TGATCAAGCG GTATGATGAG CACCATCAGG
9001     ACCTCACCTT GCTTAAGGCC CTCGTGCGTC AGCAATTGCC TGAGAAGTAT AAGGAAATCT
9061     TCTTTGACCA ATCCAAGAAC GGATACGCAG GGTATATTGA TGGCGGTGCG AGCCAGGAGG
9121     AATTCTACAA GTTTATCAAG CCGATTTTGG AGAAGATGGA CGGCACTGAG GAACTGCTCG
9181     TCAAGCTGAA TCGCGAAGAT TTGCTTCGTA AGCAACGAAC GTTCGACAAC GGCTCCATCC
9241     CGCACCAGAT TCATCTGGGC GAGCTCCACG CCATCCTTCG ACGCCAGGAA GATTTCTACC
9301     CATTTCTGAA GGACAACCGT GAGAAGATCG AAAAGATTCT TACATTCCGA ATCCCCTACT
9361     ATGTGGGACC TTTGGCCCGT GGGAATTCCC GATTTGCTTG GATGACCCGA AAGAGCGAGG
9421     AAACCATCAC TCCGTGGAAC TTCGAGGAAG TCGTGGACAA GGGTGCATCC GCGCAGAGCT
9481     TCATTGAGCG GATGACCAAT TTTGATAAGA ACCTTCCGAA TGAAAAGGTC CTGCCAAAGC
9541     ATTCGCTGCT CTACGAGTAT TTCACCGTGT ATAACGAACT GACTAAGGTC AAGTACGTGA
9601     CGGAGGGAAT GCGGAAGCCA GCCTTCCTCT CAGGGGAACA AAAGAAGGCT ATCGTCGATT
9661     TGCTTTTTAA GACCAATCGT AAAGTGACTG TTAAGCAGCT GAAGGAGGAT TATTTCAAGA
9721     AGATTGAATG TTTCGACTCC GTCGAGATCA GCGGCGTGGA AGATCGCTTT AACGCTTCCC
9781     TCGGTACCTA CCACGACCTG CTCAAGATCA TTAAGGACAA GGATTTCCTC GATAACGAGG
9841     AAAATGAGGA CATCTTGGAA GATATTGTCC TCACGTTGAC ACTTTTTGAG GACCGCGAAA
9901     TGATCGAGGA ACGGCTCAAG ACATATGCCC ATTTGTTCGA CGATAAGGTG ATGAAGCAGC
9961     TGAAGCGGCG TCGATACACC GGATGGGGTC GCCTTAGCCG GAAGCTGATC AACGGCATTC
10021    GAGATAAGCA ATCTGGTAAG ACTATCTTGG ATTTCCTTAA GTCGGACGGC TTCGCCAACC
10081    GCAATTTTAT GCAGCTTATT CACGACGATT CCCTGACGTT CAAGGAGGAC ATCCAGAAGG
10141    CACAAGTCTC AGGACAAGGG GATTCCCTGC ACGAGCATAT CGCCAACCTG GCTGGATCCC
10201    CGGCGATCAA GAAGGGGATT CTTCAGACCG TCAAGGTTGT CGACGAGCTG GTCAAGGTGA
10261    TGGGCCGTCA TAAGCCAGAA AACATCGTGA TTGAGATGGC CCGAGAAAAT CAGACCACTC
10321    AAAAGGGTCA GAAGAACAGC CGCGAGCGGA TGAAGCGGAT CGAGGAAGGC ATTAAGGAAC
10381    TTGGTTCTCA GATCCTGAAG GAGCACCCTG TTGAAAACAC ACAGCTCCAA AATGAGAAGC
10441    TGTATCTCTA CTATTTGCAA AATGGACGCG ACATGTACGT CGATCAGGAG CTCGACATTA
10501    ACCGGTTGTC GGACTACGAT GTTGACCATA TCGTCCCGCA ATCCTTCCTT AAGGACGATA
10561    GCATTGATAA CAAGGTGCTG ACTCGCTCAG ATAAGAACCG GGGCAAGTCC GACAATGTTC
10621    CAAGCGAGGA AGTGGTTAAG AAGATGAAGA ACTACTGGCG CCAATTGCTT AATGCCAAGC
10681    TCATCACACA GCGCAAGTTT GACAACTTGA CCAAGGCCGA GCGGGGAGGG CTGAGTGAAC
10741    TCGATAAGGC TGGCTTCATC AAGCGTCAAC TCGTGGAGAC GCGACAGATC ACAAAGCACG
10801    TTGCTCAGAT TCTGGACTCC CGGATGAACA CAAAGTACGA CGAGAATGAT AAGCTCATCC
10861    GTGAAGTTAA GGTCATTACC CTCAAGTCTA AGTTGGTGTC GGATTTCCGC AAGGACTTCC
10921    AATTTTATAA GGTTCGGGAG ATCAACAATT ATCACCATGC ACATGATGCG TACCTCAACG
10981    CAGTCGTGGG AACTGCGCTC ATCAAGAAGT ATCCCAAGTT GGAGTCCGAA TTCGTCTACG
11041    GGGATTATAA GGTTTACGAC GTCCGCAAGA TGATCGCCAA GAGTGAGCAG GAAATTGGCA
11101    AGGCCACGGC TAAGTATTTC TTTTACTCCA ACATCATGAA TTTCTTTAAG ACGGAGATCA
11161    CACTCGCCAA TGGAGAAATC CGTAAGCGAC CTTTGATTGA GACCAACGGC GAGACTGGTG
11221    AAATCGTTTG GGATAAGGGG CGCGACTTCG CTACCGTGCG GAAGGTTCTG AGCATGCCGC
11281    AAGTCAATAT CGTCAAGAAA ACCGAGGTGC AGACAGGCGG TTTCTCTAAG GAATCGATTC
11341    TTCCAAAGCG TAACTCTGAC AAGCTGATCG CTCGAAAGAA GGATTGGGAC CCCAAGAAGT
11401    ATGGAGGGTT CGATTCTCCT ACAGTGGCAT ACTCGGTTCT CGTTGTCGCG AAGGTTGAGA
11461    AGGGAAAGTC TAAGAAGCTG AAGTCGGTCA AGGAACTGCT CGGGATCACC ATTATGGAGC
11521    GCTCCAGCTT CGAAAAGAAT CCCATCGACT TTCTCGAGGC CAAGGGCTAT AAGGAAGTCA
11581    AGAAGGATCT TATCATTAAG CTGCCTAAGT ACTCTTTGTT CGAGCTTGAA AACGGTCGAA
11641    AGCGAATGCT CGCATCGGCA GGAGAGTTGC AGAAGGGGAA TGAATTGGCA CTTCCCTCAA
11701    AGTACGTGAA CTTCCTGTAT CTCGCGTCCC ACTACGAGAA GCTGAAGGGT AGCCCTGAGG
11761    ACAACGAACA GAAGCAACTT TTTGTTGAGC AACACAAGCA TTATCTGGAT GAGATCATTG
11821    AACAGATTTC AGAGTTCAGT AAGCGCGTCA TCCTCGCCGA TGCTAATCTC GACAAGGTGT
11881    TGTCGGCCTA CAACAAGCAC CGTGACAAGC CGATCCGAGA GCAGGCTGAA AATATCATTC
11941    ATCTGTTCAC CCTCACTAAC TTGGGAGCAC CAGCAGCGTT CAAGTATTTT GATACGACAA
12001    TCGACCGTAA GCGATACACG TCCACAAAGG AGGTGCTTGA TGCGACCCTG ATTCATCAAT
12061    CCATCACTGG GCTCTATGAA ACCCGTATCG ACCTTAGTCA ACTGGGGGGC GACCCTCCCA
12121    AGAAGAAGCG CAAGGTCTGA GCGGACATTC GATTTATGCC GTTATGACTT CCTTAAAAAA
12181    GCCTTTACGA ATGAAAGAAA TGGAATTAGA CTTGTTATGT AGTTGATTCT ACAATGGATT
12241    ATGATTCCTG AACTTCAAAT CCGCTGTTCA TTATTAATCT CAGCTCTTCC CGTAAAGCCA
12301    ATGTTGAAAC TATTCGTAAA TGTACCTCGT TTTGCGTGTA CCTTGCTTAT CACGTGATAT
12361    TACATGACCT GGACAGAGTT CTGCGCGAAA GTCATAACGT AAATCCCGGG CGGTAGGTGC
12421    GTCCCGGGCG GAAGGTAGTT TTCTCGTCCA CCCCAACGCG TTTATCAACC TCAACTTTCA
12481    ACAACCATCA TGCCACCAAA AGCGCGTAAA ACAAAGCGAG ATTTGATTGA GCAAGAGGGC
12541    AGGATCCAAT GCGCGATTCA AGACATTAAA AATGGAAAAT TTCAAAAAAT TGCGCCCGCA
12601    GCGCGTGCAT ACAAAATTCA TCCCAATACA AGTGTAATGC TAGTGGAGGT CAACACATCA
12661    ATGCCTATTT TGGTTTAGTC GTCCAGGCGG TGAGCACAAA ATTTGTGTCG TTTGACAAGA
12721    TGGTTCATTT AGGCAACTGG TCAGATCAGC CCCACTTGTA GCAGTAGCGG CGGCGCTCGA
12781    AGTGTGACTC TTATTAGCAG ACAGGAACGA GGACATTATT ATCATCTGCT GCTTGGTGCA
12841    CGATAACTTG GTGCGTTTGT CAAGCAAGGT AAGTGGACGA CCCGGTCATA CCTTCTTAAG
12901    TTCGCCCTTC CTCCCTTTAT TTCAGATTCA ATCTGACTTA CCTATTCTAC CCAAGCATCC
12961    AAATGAAAAA GCCTGAACTC ACCGCGACGT CTGTCGAGAA GTTTCTGATC GAAAAGTTCG
13021    ACAGCGTCTC CGACCTGATG CAGCTCTCGG AGGGCGAAGA ATCTCGTGCT TTCAGCTTCG
13081    ATGTAGGAGG GCGTGGATAT GTCCTGCGGG TAAATAGCTG CGCCGATGGT TTCTACAAAG
13141    ATCGTTATGT TTATCGGCAC TTTGCATCGG CCGCGCTCCC GATTCCGGAA GTGCTTGACA
13201    TTGGGGAGTT CAGCGAGAGC CTGACCTATT GCATCTCCCG CCGTGCACAG GGTGTCACGT
13261    TGCAAGACCT GCCTGAAACC GAACTGCCCG CTGTTCTCCA GCCGGTCGCG GAGGCCATGG
13321    ATGCGATTGC TGCGGCCGAT CTTAGCCAGA CGAGCGGGTT CGGCCCATTC GGACCGCAAG
13381    GAATCGGTCA ATACACTACA TGGCGTGATT TCATATGCGC GATTGCTGAT CCCCATGTGT
13441    ATCACTGGCA AACTGTGATG GACGACACCG TCAGTGCGTC CGTCGCGCAG GCTCTCGATG
13501    AGCTGATGCT TTGGGCCGAG GACTGCCCCG AAGTCCGGCA CCTCGTGCAT GCGGATTTCG
13561    GCTCCAACAA TGTCCTGACG GACAATGGCC GCATAACAGC GGTCATTGAC TGGAGCGAGG
13621    CGATGTTCGG GGATTCCCAA TACGAGGTCG CCAACATCCT CTTCTGGAGG CCGTGGTTGG
13681    CTTGTATGGA GCAGCAGACG CGCTACTTCG AGCGGAGGCA TCCGGAGCTT GCAGGATCGC
13741    CGCGCCTCCG GGCGTATATG CTCCGCATTG GTCTTGACCA ACTCTATCAG AGCTTGGTTG
13801    ACGGCAATTT CGATGATGCA GCTTGGGCGC AGGGTCGATG CGACGCAATC GTCCGATCCG
13861    GAGCCGGGAC TGTCGGGCGT ACACAAATCG CCCGCAGAAG CGCGGCCGTC TGGACCGATG
13921    GCTGTGTAGA AGTACTCGCC GATAGTGGAA ACCGACGCCC CAGCACTCGT CCGAGGGCAA
13981    AGGAATAGAG TAGATGCCGA CCGATCCCCA CTACCGCATT AAGACCTCAG CGCGGCCGCA
14041    AATTTAAATA AAATGAAGTG AAGTTCCTAT ACTTTCTAGA GAATAGGAAC TTCTATAGTG
14101    AGTCGAATAA GGGCGACACA AAATTTATTC TAAATGCATA ATAAATACTG ATAACATCTT
14161    ATAGTTTGTA TTATATTTTG TATTATCGTT GACATGTATA ATTTTGATAT CAAAAACTGA
14221    TTTTCCCTTT ATTATTTTCG AGATTTATTT TCTTAATTCT CTTTAACAAA CTAGAAATAT
14281    TGTATATACA AAAAATCATA AATAATAGAT GAATAGTTTA ATTATAGGTG TTCATCAATC
14341    GAAAAAGCAA CGTATCTTAT TTAAAGTGCG TTGCTTTTTT CTCATTTATA AGGTTAAATA
14401    ATTCTCATAT ATCAAGCAAA GTGACAGGCG CCCTTAAATA TTCTGACAAA TGCTCTTTCC
14461    CTAAACTCCC CCCATAAAAA AACCCGCCGA AGCGGGTTTT TACGTTATTT GCGGATTAAC
14521    GATTACTCGT TATCAGAACC GCCCAGGGGG CCCGAGCTTA AGACTGGCCG TCGTTTTACA
14581    ACACAGAAAG AGTTTGTAGA AACGCAAAAA GGCCATCCGT CAGGGGCCTT CTGCTTAGTT
14641    TGATGCCTGG CAGTTCCCTA CTCTCGCCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT
14701    CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA
14761    CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA
14821    ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC
14881    ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG
14941    CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT
15001    ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT
15061    ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC
15121    AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG
15181    ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG
15241    GTGCTACAGA GTTCTTGAAG TGGTGGGCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG
15301    GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG
15361    GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA
15421    GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA
15481    ACGACGCGCG CGTAACTCAC GTTAAGGGAT TTTGGTCATG AGCTTGCGCC GTCCCGTCAA
15541    GTCAGCGTAA TGCTCTGCTT