乙酰化磷酸化甲基化抗体定制技术服务_详细资料

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克隆性：多克隆规格： ml

上海睿星基因技术有限公司从2001年开始为科研人员提供单克隆/多克隆抗体制备技术服务，从2006年起和复旦大学管坤良&熊跃实验室（MCB lab）合作制备乙酰化抗体，磷酸化抗体，甲基化抗体，MCB lab发表文章列表如下。上海睿星基因技术有限公司在修饰抗体制备方面积累了大量的经验，为国内外的科研人员制备了大量的位点特异性修饰抗体。

抗体制备技术服务部分客户单位：复旦大学；浙江大学；清华大学；军事医学科学院；
  UCSD；Emory University；Novartis；Wako

ShanghaiGenomics provided Customized antibody production service to lifescience researchers from 2001,we can generate the rabbit polyclonal antibody and mouse monoclonal antibody for customers. 17 years experience of Customized antibody production service and commercial antibody products R&D can make our service and antibody products perfect.
We have 12 years experience of site specific antibody production,eg Actylation, phosphorylation,Methylation.Our company collaborate with MCB lab Fudan University for site specific antibody production from 2006.The MCB lab has published 47 research papers from 2008.

Publication is listed as below,

http://www.mcb.fudan.edu.cn/ MCB lab（esdablished in 2006） PI：Kunliang Guan & Yue Xiong
1 .EMBO Rep.
Chen, X-F., Tian, M-X., Sun, R-Q., Zhang, M-L., Zhou, L-S., Jin, L., Chen, L-L., Zhou, W-J., Duan, K-L., Chen, Y-J., Gao, C., Cheng, Z-L., Wang, F., Zhang, J-Y., Sun, Y-P., Yu, H-X., Zhao, Y-Z., Yang, Y., Liu, W-R., Shi, Y-H., Xiong, Y., Guan, K-L., Ye, D.. (2018) SIRT5 inhibits peroxisomal ACOX1 to prevent oxidative damage and is downregulated in liver cancer. EMBO Rep. Feb 28. pii: e45124. doi: 10.15252/embr.201745124.
Antibodies specific to Flag (Shanghai genomic)

Li, F-L., Liu, J-P., Bao, R-X., Yan, G., Feng, X., Xu, Y-P., Sun, Y-P., Yan, W., Ling, Z-Q., Xiong, Y., Guan, K-L., and Yuan, H-X. (2018) Acetylation accumulates PFKFB3 in cytoplasm to promote glycolysis and protects cells from cisplatin-induced apoptosis. Nat Commun, 9:508.
To generate a site-specific antibody to detect the acetylated K472 of PFKFB3 (anti-acK472, 1:500), synthesized peptide SPEPTK(Ac)KPRINS (Shanghai Genomic Inc.) was coupled to KLH as antigen to immunize rabbit. Anti-serum was collected after four doses of immunization.

3. Lv, L., Xu, Y-P., Zhao, D., Li, F-L., Wang, W., Sasaki N., Jiang, Y., Zhou, X., Li, T-T., Guan, K-L.,Lei, Q-Y.,and Xiong, Y.,(2013) Mitogenic and oncogenic stimulation of K433 acetylation promote PKM2 protein kinase activity and nuclear localization. Molecular Cell , 2013, 52, 340–352
prepare the PKM2 K433 acetylation antibody

4. Lin,R., Ren, T., Gao, X., Zhou, X., Li, T., Xiong, Y., Guan, K-L., Lei, Q-Y..(2013) Acetylation Stabilizes ATP-citrate Lyase to Promote Lipid Biosynthesis and Tumor Growth. Molecular Cell 51, 506–518
Antibodies specifically recognizing acetylation at lysines 540, 546, and 554 (Ac[3K]) were prepared commercially by immunizing rabbits at Shanghai Genomics, Inc.

5. Han, X-R., Zha, Z-Y., Yuan, H-X., Feng, X., Xia, Y-K., Lei, Q-Y., Guan, K-L., and Xiong, Y., (2016) KDM2B/FBXL10 targets c-Fos for ubiquitylation and degradation in response to mitogenic stimulation. Oncogene, 35: 4179-4190.
Antibodies and immunological procedures
Antibodies against FLAG (SG4110-16; Shanghai Genomics Technology ), MYC (SG4110-18; Shanghai Genomics Technology)

6. Zhou, L-S., Wang, F., Sun, R-Q., Chen, X-F., Zhang, M-L.,Xu, Q., Wang, Y., Wang, S-W., Xiong, Y., Guan, K-L.,Yang, P-Y., Yu, H-X., Ye, D.,(2016).SIRT5 promotes IDH2 desuccinylation and G6PD deglutarylation to enhance cellular antioxidant defense. EMBO Rep. 17(6):811-22.
Flag (Shanghai Genomics, 4110-20) were purchased commercially.

7. Wang, P., Wu, J., Ma, S-H., Zhang, L., Yao, J., Hoadley, KA., Wilkerson MD., Perou CM., Guan, K-L., Ye, D., Xiong, Y., (2015).Oncometabolite D-2-Hydroxyglutarate Inhibits ALKBH DNA Repair Enzymes and Sensitizes IDH Mutant Cells to Alkylating Agents. Cell Rep. 13(11):2353-61
Antibodies against Flag (ShanghaiGenomics)

Lv, X-B., Liu, C-Y., Wang, Z., Sun, Y-P., Xiong, Y., Lei, Q-Y., Guan, K-L.,(2015) PARD3 Induces TAZ Activation and Cell Growth by Promoting LATS1 and PP1 Interaction. EMBO Rep. 16(8):975-85.
Antibodies to TAZ and to phospho-TAZ (S89) were generated by immunizing rabbits with indicated antigen peptides at Shanghai Genomic Inc.

Wang, S-W., Jiang, B-W., Zhang, T-F., Liu, L-X., Wang, Y., Wang, Y-P., Chen, X-F., Lin, H-P., Zhou, L-S., Xia, Y-K., Chen, L-L., Yang, C., Xiong, Y., Ye, D., Guan, K-L.(2015) Insulin and mTOR Pathway Regulate HDAC3-Mediated Deacetylation and Activation of PGK1. PLoS Biol.13(9):e1002243. doi: 10.1371
To generate acetyl-lysine 220 specific antibody of PGK1, synthetic peptide VADKIQLINNMLDK was coupled to KLH as antigen to immunize rabbit (Shanghai Genomic Inc).

http://www.cancermetabolism.fudan.edu.cn/Content.aspx?infolb=26&flag=2
Cancer metabolism lab PI：Qunying Lei

10. Wang YP*, Zhou W, Wang J, Huang X, Zuo Y, Wang TS, Gao X, Xu YY, Zou SW, Liu YB, Cheng JK*, Lei QY*. Arginine Methylation of MDH1 by CARM1 Inhibits Glutamine Metabolism and Suppresses Pancreatic Cancer. Molecular Cell 2016, 64: 673–687.   Highlights：Cancer discovery
MDH1 R248 Arginine Methylation antibody

11. Li T, Liu M, Feng X, Wang Z, Das I, Xu Y, Zhou X, Sun Y, Guan KL, Xiong Y, Lei QY*. Glyceraldehyde-3-phosphate Dehydrogenase Is Activated by Lysine 254 Acetylation in Response to Glucose Signal. J Biol Chem. 2014, 289(6):3775-3785.
Glyceraldehyde-3-phosphate Dehydrogenase Lysine 254 antibody

南昌大学生科院&医学院王建斌实验室
12.Tianyu Han1,2, Weihua Zhan1,2, Mingxi Gan1, Fanrong Liu3, Bentong Yu4, Y. Eugene Chin5and Jian-Bin Wang. Phosphorylation of glutaminase by PKCεis essential for itsenzymatic activity and critically contributes to tumorigenesis.Cell Research (2018) 0:1–15
The anti-phospho-serine 314 speciﬁcpolyclonal antibody against GAC (GAC-pS314) was made by Shanghai Genomics Inc

徐彦辉实验室复旦大学生物医学研究院
13.Xizi Chen, Mengjie Liu, Yuan Tian, Jiabei Li, Yilun Qi, Dan Zhao, Zihan Wu, Min Huang, Catherine C. L. Wong, Hong-Wei Wang, Jiawei Wang, Huirong Yang* & Yanhui Xu*. Cryo-EM structure of human mTOR complex 2. Cell Research. Published online 22 March 2018
Anti-Myc HRP（GNI4310-MC-S, GNI Group）

After 3 years R&D experiments,Our lab developed the best anti-flag monoclonal antibody around the world in 2004.It remains the best anti-flag monoclonal antibody until now. We sold more than 4000ml anti-flag affinity gel around the world in 2017.We have tag series antibody ,internal contral antibody(β-Actin,GAPDH，β-tubulin,α-tubulin) and anti-tag affinity gel (flag HA Myc V5).
We provide excellent customized monoclonl antibody service to customers from 2004，we collaborate with Novartis for Mab in 2007.

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