bacteriophage Pf1

如需订购请联系郭经理    订购电话：15910316995   订购QQ：3579764566     PF1 filamentous phage Co-solvent for NMR applications Cat No.: BioVector690317 Description Pf1 is a filamentous bacteriophage, propagated on Pseudomonas aeruginosa Kr. Filamentous phages can be employed in nuclear magnetic resonance (NMR) applications as co-solvents for dipolar coupling experiments. Application in a wide temperature range and ease of phage ñ sample ñ separation or buffer exchange make Pf1 filamentous phage a superior co-solute for NMR experiments. Form Filamentous phage Pf1 (min. 50 mg/ml) in 10 mM Potassium Phosphate, pH 7.6, 2 mM MgCl₂, 0.02 % NaN3 Storage Pf1 filamentous phage must be stored at 0 to 4°C. Do not Freeze. The phage is stable at 0 to 4°C until the expiry date indicated on the vial. Technical Note Buffer exchange: Buffer can be changed by either ultrafiltration membranes of MWCO 30 kDa or less (e.g. Millipore ZM 30), or by ultracentrifugation. For ultracentrifugation, spin the phage e.g. at 40.000 rmp for 6 h in a Beckman Ti 50 rotor. Note: Phage recovery after buffer exchange may vary, and recovery of phage may be less than 70 %. Specifications Dimensions of Pf1 (average): 2000 x 6 nm. Security advices: Avoid proteases. Since Pf1 solution is highly viscous at the concentration at hand, use caution in pipetting.  Briefly spin down the phage solution prior to withdrawal, as Pf1 may be distributed across the entire vial.  However, use precaution handling the phage material.  Pf1 was isolated from Pseudomonas aeruginosa Kr, a potential pathogen. No health risk should emanate from highly purified Pf1 phage.  NaN₃ is highly toxic (R28 ñ 32; S 28 - 45). Wear goggles and gloves when handling the phage solution. References: I. Markus MA, Gerstner RB, Draper DE, Torchia DA. Refining the overall structure and subdomain orientation of ribosomal protein S4 delta41with dipolar couplings measured by NMR in uniaxial liquid crystalline phases. J Mol Biol. 1999 Sep 17;292(2):375-87. II. Zweckstetter M, Bax a. Characterization of molecular alignment in aqueous suspensions of Pf1 bacteriophage. J Biomol NMR. 2001 Aug;20(4):365-77 III. Hansen MR, Mueller L, Pardi A. Tunable alignment of macromolecules by filamentous phage yields dipolar coupling interactions. Nat Struct Biol. 1998 Dec;5(12).1065-74.