大鼠糖原合成酶激酶3β(GSK3B)ELISA kit/ Rat Glycogen synthase kinase-3 beta(GSK3B) ELISA kit_详细资料

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品信息 样本： Rat 标记物： glycogen synthase kinase 3 beta 适应物种： Rat 应用： Metabolism 检测限： 0.156 ng/ml-10 ng/ml 供应商：北京爱特蒙科技有限公司数量： 100 规格： 96T 产品类型: ELISA Kit 产品名称: 大鼠糖原合成酶激酶3β(GSK3B)ELISA kit 英文名称: Rat Glycogen synthase kinase-3 beta(GSK3B) ELISA kit 货号: CSB-EL009963RA 别名: , GSK3beta isoform 规格: 96T 中文价格: 3600 种属: Rat 待测物名称: glycogen synthase kinase 3 beta 缩写: GSK3B 蛋白功能1: Biosynthesis/Metabolism 蛋白功能2: glyconeogenesis and glycometabolism 蛋白功能3: Carbohydrate metabolism 检测范围: 0.156 ng/ml-10 ng/ml 灵敏度: 0.039 ng/ml 反应时间: 1-5h 所需样本体积: 50-100ul 检测波长: 450 nm 标准曲线: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 10 2.241 2.356 2.299 2.147 5 1.544 1.648 1.596 1.444 2.5 1.022 0.992 1.007 0.855 1.25 0.661 0.654 0.658 0.506 0.625 0.423 0.432 0.428 0.276 0.312 0.307 0.315 0.311 0.159 0.156 0.228 0.224 0.226 0.074 0 0.151 0.152 0.152 精密度: Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. 线性度: To assess the linearity of the assay, samples were spiked with high concentrations of rat GSK3B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 92 Range % 86-96 1:2 Average % 102 Range % 97-104 1:4 Average % 95 Range % 89-99 1:8 Average % 97 Range % 92-104 回收率: The recovery of rat GSK3B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 94 89-97 EDTA plasma (n=4) 98 92-102 研究领域: Metabolism 引用文献: 1. Developmental perfluorooctane sulfonate exposure results in tau hyperphosphorylation and 尾-amyloid aggregation in adults rats: Incidence for link to Alzheimer's disease , Zhang Q.et al,Toxicology.,2016,347-349:40-6. 2. Mechanism of Sphingosine-1-Phosphate induced cardioprotection against I/R injury in diabetic rat heart: Possible involvement of Glycogen synthase kinase 3尾 and mitochondrial permeability transition pore. , Rana A. et al,Clin Exp Pharmacol Physiol,2015,19 3. Neuroprotective effect of hemeoxygenase-1/glycogen synthase kinase-3β modulators in 3-nitropropionic acid-induced neurotoxicity in rats , Khan A.et al,Neuroscience.,287:66-77 资料下载： CSB-EL009963RA_Rat_Glycogen_synthase_kinase-3_beta(GSK3B)_ELISA_kit.pdf 附件下载 (下载 0 次) 请 [登录] 后再下载!

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To assess the linearity of the assay, samples were spiked with high concentrations of rat GSK3B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-96
1:2	Average %	102
	Range %	97-104
1:4	Average %	95
	Range %	89-99
1:8	Average %	97
	Range %	92-104

The recovery of rat GSK3B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-97
EDTA plasma (n=4)	98	92-102