abcam免疫学抗体人Inflammation抗体芯片_详细资料

产品信息

抗体英文名：人Inflammation抗体Array -膜(20 targets) 克隆性：多克隆

样品类型
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media

检测类型Semi-quantitative

种属反应性
与反应: Human

存放说明Store at -20°C. Please refer to protocols.

Human Inflammation Antibody Array - Membrane (20 targets) 图像

Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (20 targets) (ab169804)

Human peripheral blood cells (1x10⁶ cells/mL) were cultured in RPMI media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 mg/mL streptomycin sulfate.

Cells were cultured unstimulated or stimulated with 10 mg/mL PHA. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab169804. Media alone was used as a negative control.

Quantification of Human Inflammation Antibody Array

Cells were cultured unstimulated or stimulated with 10 mg/mL PHA. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab169804. Media alone was used as a negative control. Mean pixel density was quantified using CCD camera software analysis.

Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (20 targets) (ab169804)

HepG2 cells (Human hepatocellular carcinoma) were seeded at 1x10⁶ cells/mL and cultured in EMEM media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 µg/mL streptomycin sulfate. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab169804.

Quantification of Human Inflammation Antibody Array

Conditioned media was harvested after 48 hours, aliquoted and assayed using ab169804. Mean pixel density was quantified using CCD camera software analysis.

Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (20 targets) (ab169804)

Typical results obtained with Abcam Membrane Antibody Arrays. Image above presents typical images obtained with Abcam Human Cytokine Membrane Antibody Array. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at RT for 1 min.

组件 1 x 4 Membranes 1 x 8 Membranes

1000X HRP-Conjugated Streptavidin 1 x 50µl 1 x 50µl

1X Blocking Buffer 1 x 25ml 1 x 50ml

2000X Biotin-Conjugated Anti Cytokines 2 vials 4 vials

20X Wash Buffer I 1 x 10ml 1 x 20ml

20X Wash Buffer II 1 x 10ml 1 x 20ml

2X Cell Lysis Buffer 1 x 10ml 1 x 20ml

8-Well Plastic Tray 1 unit 1 unit

Detection Buffer C 1 x 1.5ml 1 x 2.5ml

Detection Buffer D 1 x 1.5ml 1 x 2.5ml

Inflammation Antibody Array Membranes 4 units 8 units

组件	1 x 4 Membranes	1 x 8 Membranes
1000X HRP-Conjugated Streptavidin	1 x 50µl	1 x 50µl
1X Blocking Buffer	1 x 25ml	1 x 50ml
2000X Biotin-Conjugated Anti Cytokines	2 vials	4 vials
20X Wash Buffer I	1 x 10ml	1 x 20ml
20X Wash Buffer II	1 x 10ml	1 x 20ml
2X Cell Lysis Buffer	1 x 10ml	1 x 20ml
8-Well Plastic Tray	1 unit	1 unit
Detection Buffer C	1 x 1.5ml	1 x 2.5ml
Detection Buffer D	1 x 1.5ml	1 x 2.5ml
Inflammation Antibody Array Membranes	4 units	8 units

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