EpiNext 高通量DNA纯化系统(磁珠法) 货号:A-P-1063
EpiNext DNA Purification HT System
背景资料:Obtaining high recovery of purified DNA or selected DNA fragments is critical for downstream applications that use DNA samples including PCR, sequencing, cloning, microarray, and DNA fragment analysis, regardless of the platform used.
产品描述: DNA fragments of various lengths. Input amount can be from 0.1 ng to 1 µg. The bead volumes provided in the system of 4 ml or 8 ml permit a total of 96 or 192 standard purifications, respectively (use 0.5 µg of DNA in 20 µl of solution); or 48 or 96 standard DNA size selections, respectively (use 50 µl of input DNA solution).
效果展示: 图a | 图b |
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Gel picture after purification of 800 ng of DNA marker (Hyperladder 50 bp ladder, Bioline) using the EpiNext™ DNA Purification HT System with varying ratios of beads from 0.9X to 2.1X. AMPure XP beads were used as the control. M: Marker size of Agilent DNA ChIP kit | Percentage of recovery of input DNA fragments by "AMPure XP" beads vs “EpiNext” beads: 780 ng of DNA marker (Hyperladder 50 bp ladder, Bioline) were purified using the EpiNext™ DNA Purification HT System at 1.8X. Agencourt AMPure XP beads (1.8X) were used as the control. Recovery yield of 100 bp or higher DNA fragments was quantified with an Agilent Bioanalyzer 2100 |
产品特点:1·
Optimized buffer chemistries: Complete separation of DNA or PCR amplicons. It can also be used for DNA size selection based on the ratio of beads to DNA sample volume;
2·
Fast and straightforward: The entire procedure can be finished in just 30 min for 96 samples and is highly amenable to a variety of automation platforms. No gels, columns, filtration, or centrifugation is needed;
3.
Efficient clean-up: Removal of excess primers, adaptors, nucleotides, salts, enzymes, and PCR inhibiting substances such as polysaccharides, polyphenols, lipids and dyes;
4.
High recovery of DNA: Higher than 85% recovery of input DNA;
5.
Manual and automation friendly: Scalable for use in single tube or 96-well plate formats;
6.
Ultimate value: Superior performance for less than half the price vs Agencourt® AMPure® XP beads, without any having to change your existing workflows。
保存建议:在接收到艾德寄出的试剂盒后请按照说明书建议,使用不同的保存条件或温度来保存试剂盒内组分。
定购信息: 产品名称 | 规格 | 操作手册 | 货号 | 询价 |
EpiNext 高通量DNA纯化系统(磁珠法) | 4 ml | PDF | A-P-1063-04 | |
EpiNext 高通量DNA纯化系统(磁珠法) | 8 ml | PDF | A-P-1063-08 | |
EpiNext 高通量DNA纯化系统(磁珠法) | 64 ml | PDF | A-P-1063-64 | |
EpiNext 高通量DNA纯化系统(磁珠法) | 480 ml | PDF | A-P-1063-X4 | |
EpiNext 高保真cDNA 链合成试剂盒 | 20 次 | 英文PDF-中文PDF | A-P-9004 | |
A&DNext 高保真cDNA链合成试剂盒 | 50 次 | PDF | A-P9006 | |
组织总RNA提取试剂盒 | 50 次 | PDF | A-RH114-01 | |
组织总RNA提取试剂盒 | 200 次 | PDF | A-RH114-02 | |
组织/细胞总RNA提取试剂盒II | 50 次 | PDF | A-RH113-01 | |
组织/细胞总RNA提取试剂盒II | 200 次 | PDF | A-RH113-02 | |
血液总RNA快速提取试剂盒II | 50 次 | PDF | A-RH716-01 | |
血液总RNA快速提取试剂盒II | 200 次 | PDF | A-RH716-02 | |
多糖多酚植物总RNA快速提取试剂盒(离心柱型) | 50 次 | PDF | A-R3202 | |
通用植物总RNA大量提取试剂盒(离心柱) | 10 次 | PDF | A-R3312 | |
血液总RNA提取试剂盒(离心柱型) | 100次 | PDF | A-R4002 | |
表观遗传学产品全面解决方案列下: 产品名称 | 规格 | 操作手册 | 货号 | 询价 |
DNA羟甲基化定量试剂盒(荧光法) | 48 次 | PDF | A-P-1037-48 | |
DNA羟甲基化定量试剂盒(荧光法) | 96 次 | PDF | A-P-1037-96 | |
DNA羟甲基化定量试剂盒(比色法) | 48 次 | PDF | A-P-1036-48 | |
DNA羟甲基化定量试剂盒(比色法) | 96 次 | PDF | A-P-1036-96 | |
DNA甲基化定量试剂盒(荧光法) | 48 次 | PDF | A-P-1035-48 | |
DNA甲基化定量试剂盒(荧光法) | 96 次 | PDF | A-P-1035-96 | |
DNA甲基化定量试剂盒(比色法) | 48 次 | PDF | A-P-1034-48 | |
DNA甲基化定量试剂盒(比色法) | 96 次 | PDF | A-P-1034-96 | |
通用DNA甲基化定量试剂盒 | 48 次 | PDF | A-P-1011-48 | |
通用DNA甲基化定量试剂盒 | 96 次 | PDF | A-P-1011-96 | |
超敏DNA甲基化定量试剂盒 | 48 次 | PDF | A-P-1021-48 | |
超敏DNA甲基化定量试剂盒 | 96 次 | PDF | A-P-1021-96 | |
总DNA甲基化定量试剂盒 | 48 次 | PDF | A-P-1014-48 | |
总DNA甲基化定量试剂盒 | 96 次 | PDF | A-P-1014-96 | |
推荐阅读:关于5-hmC
5-hmC是近年来在动物组织中发现的,由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能,有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息,也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前,我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序,这些技术都不适合用来检测5-hmC,因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题,EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶,并且区分5-hmC, 5-mC, 和 C,使得研究者能够重新评估他们的DNA甲基化信息,也能够在新样品中寻找DNA羟甲基化。
温馨提示:不可用于临床ZL。