Bulk HCV Core Antigen Detection ELISA Assay, 10 ELISA Plates

The reagents supplied in this pack are for Research use only.
1 Coated microwell strips.
Plastic microtitration wells coated with
anti-HCV Core Ag antibody in foil pouch
with desiccant. 1 plate(96 wells)
2 Positive Core Antigen Control 100 ng/ml 0.1 mL
3 Lysis Buffer 6 mL
4 Conjugate. HRP Conjugated anti-HCVCore Antibody. 12 mL
5 Wash Buffer (20x concentrated). Tris
buffered saline pH 7.8-8.0, containing
0.05% Tween 20. Must be diluted
before use. 2 Bottles60 mL
6 Substrate Solution. Ready to use.
Tetramethylbenzidine 12 mL
7 Stop Solution. 1 N H2SO4 12 mL
Additional Requirements for Manual Processing
> Disposable tip micropipettes to deliver volumes of 5 μL, 10μL,25μL, 100μL and 200μL (multichannel pipette preferred for dispensingreagents intomicrotiter plates).
> Distilled or deionized water.
> 37 (±1)º C incubator.
> Clean, disposable plastic/ glass test tubes, approximatecapacities 5 mL
and 10 mL.
> Range of standard, clean volumetric laboratory glasswareconsisting of, at
least, 15 mL and 100 mL beakers, 1 L graduated cylinder,1 mL, 5 mL, and 10
mL glass pipettes.
> Absorbent paper towels.
> Automatic microtitration plate washer or laboratory wash bottle.
> Microtitration plate reader with 450 nm filter.
> Latex gloves, safety glasses and other appropriate protective garments.
> Biohazard infectious waste containers.
> Safety pipetting devices for 1 mL or larger pipettes.
> Timer.

Storage and Stability
All reagents except for the positive control standard should be storedat2-8℃, and should not be used beyond the expiration date on thelabel.Once opened, microtitration strips may be stored at 2-8℃ until theexpirationdate on the label, provided that desiccated conditions aremaintained.Unused strips should be returned to their original foil pouchalong with thesachet of desiccant. Opened pouches should be securelyresealed by foldingover the open end and securing it with adhesive tape. I
The working strength Wash Buffer should not be stored for longer than3weeks at 2-8℃. It is recommended that Wash buffer be freshly dilutedbeforeeach assay. If the working strength buffer becomes visibly cloudyordevelops precipitate during the 3 weeks, do not use it.The positivecontrol standard should be stored frozen at -70°C. It may befreezethawed up to 5 times.