武汉纽斯特生物技术有限公司 NewEast Biosciences Configuration-specific Monoclonal Antibody Based

Product Description
Small GTPases are a super-family of cellular signaling regulators. Ras belongs to the Ras sub-family of
GTPases that regulate cell growth, cell motility, and gene transcription. GTP binding increases the
activity of Ras, and the hydrolysis of GTP to GDP renders it inactive.
Currently the activation of Ras proteins is assayed with the binding of GTP-bound Ras to the Ras-binding
domain (RBD) of Raf protein kinase. This method is based on the observation that the active,
GTP-bound Ras could bind to the RBD of Raf. However, the reproducibility of this method is poor.
This is partially due to the relatively quick hydrolysis of GTP to GDP during the assay procedure, and the
low binding affinity of RBD to Ras-GTP.
NewEast Biosciences Ras Activation Assay Kit is based on the configuration-specific monoclonal
antibody that specifically recognizes Ras-GTP, but not Ras-GDP. Given the high affinity of
monoclonal antibodies to their antigens, the activation assay could be performed in a much shorter time.
This assay provides the reliable results with consistent reproducibility.
These anti-Ras-GTP monoclonal antibody can also be used to monitor the activation of Ras in cells and
in tissues by immunohistochemistry.
NewEast Biosciences Ras Activation Assay Kit provides a simple and fast method to monitor the
activation of Ras. Each kit provides sufficient quantities to perform 20 assays.
Assay Principle
NewEast Biosciences Ras Activation Assay Kit bases on the configuration-specific anti-Ras-GTP
monoclonal antibody to measure the active Ras-GTP levels, either from cell extracts or from in vitro
GTPγS loading Ras activation assays. Briefly, anti-active Ras mouse monoclonal antibody will be
incubated with cell lysates containing Ras-GTP. The bound active Ras will then be pulled down by
protein A/G agarose. The precipitated active Ras will be detected by immunoblot analysis using anti-Ras
mouse monoclonal antibody.