产品具体信息请参见:
http://www.yrgene.com/goods-122975.html货号:VPI0016
载体名称:pTracer-CMV2
出品公司:Invitrogen
载体用途:哺乳动物表达载体
价格:1200元规格:一管质粒,5ug。附送一管甘油菌,500ul。
到货周期:现货
原核抗性:Ampicillin(氨苄青霉素)
真核筛选标记:Zeocin(博莱霉素)
启动子:CMV promter
表达水平:高
5' 测序引物:CMV-f(通用测序引物,测序公司免费提供)
3' 测序引物:BGH-r(通用测序引物,测序公司免费提供)
载体拷贝数:高
载体描述:
pTracer-CMV2是一个双表达框载体,CMV启动子后有MCS供插入外源基因;hEF1α启动子调控Cycle 3 GFP-Zeocin fusion的表达,可以做到既有荧光蛋白来做转染指示,且荧光基因表达和外源基因表达又不会互相干扰,而且Zeocin也可以用来做稳定性细胞筛选。另外,由于EM-7启动子的存在,Cycle 3 GFP-Zeocin fusion在E.coli中也可以表达,可以用来做原核荧光定位。
Three pTracer mammalian expression vectors are available that express GFP fused to the selectable marker Zeocin. Each vector uses a different set of promoters to express the gene of interest and the Cycle 3 GFP-Zeocin fusion.
All three pTracer vectors have the following features:
(1)Cycle 3 GFP-Zeocin fusion for selection in mammalian cell lines
(2)BGH polyA signal and transcription termination sequences to enhance mRNA stability
The pTracer-CMV2 vector offers:
(1)CMV promoter for high-level constitutive expression of your gene of interest
(2)EF-1α promoter for high-level constitutive expression of the Cycle 3 GFP-Zeocin fusion
(3)Ampicillin resistance gene for selection in E. coli
产品说明书及载体序列:
请访问以下页面下载【pTracer-CMV2】产品说明书及载体序列:
http://www.yrgene.com/goods-122975.html
质粒图谱:
温馨提示:不可用于临床ZL。