pLOX-CWBmi1永生化细胞系慢病毒载体
Gene/insert name:Bmi1
Species:M. musculus (mouse)
Entrez Gene:Bmi1 (RP23-396N6.2, AW546694, Bmi-1, Pcgf4)
Mutation:CMV promoter upstream of mBmi-1. LoxP in 3' LTR.
Vector backbone:pHR(Search Vector Database)
Vector type:Mammalian Expression, Lentiviral, Cre/Lox
5' sequencing primer:CMV fwd List of Sequencing Primers
3' sequencing primer:SP6 for LoxP site
Bacterial resistance(s):Ampicillin
Growth strain(s):Stbl3
Growth instructions:Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C
High or low copy:High Copy
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慢病毒载体
SBI :
pCDH-EF1-MCS-T2A-Puro
pCDH-EF1-MCS-T2A-Puro
Addgene :
pLVTHM
pLOX-CW-CRE
慢病毒包装载体
Invitrogen:
Vectors:pLp1,pLp2,pLp VSV G
Strain:Stbl3,Stbl4
Cell biolabs:
Vectors:pCgpV
Clontech:
Vector:pVSV G
Addgene
Second generation:
Vectors:pCMV-dR8.91,pCMV-VSV-G
Second generation:
Vectors:pMD2.G,pSPAX2
Third generation:
Vectors:pMDLg pRRE,pRSV-rev,pCMV-VSV-G
iPS慢病毒载体
Cell biolabs:
Vectors:pLentG-KOSM
Addgene:
Vectors:FUW-M2rtTA,TetO-FUW-OSKM
SBI (FIV):
Vectors:pCDF1-MCS2-EF1-copGFP,pFP93
建永生化细胞系慢病毒载体
Addgene:
Vector:pLOX-CWBmi1,pLOX-TERT-iresTK,pLOX-Ttag-iresTK
PiggyBac转座子系统
Vectors:HA-mPB(),PiggyBac 5’LTR (pMD18T vector),()
PiggyBac 3’LTR (pMD18T vector)()
SBI:
Vectors:PiggyBac Dual promoter (PB513B-1),Super PiggyBac Transposase (PB200PA-1)
慢病毒系统:
RNAi载体
Addgene:
Vectors:pSicoR,pSicoR pGK puro,pLentilox 3.7,pLKO.1 puro,pLKO.3G,
pPRIME-TET-GFP-FF3,pPRIME-TREX-GFP-FF3
Clontech:
Vectors:pLVX-shRNA1,pLVX-shRNA2
SBI:
Vectors:pSIH1-H1-CoGFP
Open Biosystems:
Vectors:pGIPZ empty,pGIPZ control,pTRIPZ empty,pTRIPZ control