Monoclonal ANTI-FLAG® M2 antibody produced in mouse

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

For highly sensitive and specific detection of FLAG fusion proteins by immunoblotting, immunoprecipitation, immunohistochemisty, and immunocyotchemistry. Optimized for single banded detection of FLAG fusion proteins in mammalian, plant, and bacterial expression systems.

Browse additional application references in our FLAG^® Literature portal.

Features and Benefits

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General description

The ANTI-FLAG M2 mouse, affinity purified monoclonal antibody binds to fusion proteins containing a FLAG peptide sequence.The antibody recognizes the FLAG peptide sequence at the N-terminus, Met-N-terminus,C-terminus, and internal sites of the fusion protein.

Immunogen

FLAG; peptide sequence DYKDDDDK

Preparation Note

Immediately before use, dilute in Tris buffered saline (TBS), pH 8.0, with 3% nonfat Milk

Specificity

Binding site:N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C

Physical form

Contains 50% glycerol in phosphate buffered saline.

法律信息

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC

FLAG is a registered trademark of Sigma-Aldrich Co. LLC