E7600S NEBNext Multiplex Oligos for Illumina 现货促销

The NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) contains the adaptor and index primers that are ideally suited for multiplex sample preparation for next-generation sequencing on the Illumina platform. Each of these components must pass rigorous quality control standards and are lot controlled, both individually and as a set of reagents.


 

Functional Validation

Each set of reagents is functionally validated together through construction and sequencing of a transcriptome library and sequenced on a .

Lot Control

The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page

Reagents Supplied

The following reagents are supplied with this product:
 

Materials Required but not Supplied

Required Materials Not Included for DNA or ChIP Libraries:

• Enzymes and buffers appropriate for DNA or ChIP Illumina library preparation. (DNA: #E7370, #E6040, #E6000 ChIP: #E7370, #E6240, #E6200)
• Agencourt® AMPure® XP Beads (Beckman Coulter, Inc. #A63881)
• Magnetic Stand
• DNA LoBind® Tubes (Eppendorf®) / PCR Tubes/96-well PCR Plates
• Freshly prepared 80% Ethanol
• Nuclease-free Water
• 0.1X TE (or 10 mM Tris-HCl, pH 8.0)
• 10 mM NaCl
• Bioanalyzer® (Agilent Technologies, Inc.) 3
• Bio-Rad® Microseal® (Bio-Rad Laboratories, Inc.) "A" Film or equivalent

Recommended products not supplied:

• TruSeq® Index Plate Fixture (Illumina #FC-130-1005) or similar setup (see page 9 on the manual)

Required Materials Not Included for RNA Libraries:

• Enzymes and buffers appropriate for RNA Illumina library preparation. (#E7420, #E7530, #E6110, #E6100)
• NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490)
• Magnetic Rack (Alpaqua, cat #A001322 or equivalent)
• 80% Ethanol (freshly prepared)
• 0.1X TE (or 10 mM Tris-HCl, pH 8.0)
• 10 mM NaCl
• Agencourt AMPure XP Beads (Beckman Coulter, Inc. #A63881)
• Actinomycin D (Sigma# A1410, dissolved in dimethylsulfoxide [DMSO] to 5 μg/µl).
• DNA LoBind Tubes/PCR Tubes/96-well PCR Plates
• Bioanalyzer
• Bio-Rad Microseal "A" Film or equivalent

Recommended products not supplied:

• TruSeq Index Plate Fixture (Illumina #FC-130-1005) or similar setup (see page 64 on the manual). 
• Additional materials required for use with #E6110 and #E6100:
• 3 M Sodium Acetate, pH 5.5
• Ethanol
• PCR Column Purification Kit (Qiagen or other)
• DNA Gel Extraction Column Purification Kit
• RNeasy® MinElute® Clean Up Kit (Qiagen® #74204)

Storage Temperature

-20°C

1. Do NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1)come with library prep reagents?

2. Can I use NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) for library prep from both DNA and RNA samples?

3. How many libraries can I prepare with the NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1)?

4. For pooling <96 samples, what combination of indices should I use?

5. Do I need to spike in custom sequencing primers when sequencing libraries made with NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) on the Illumina platforms?

6. Can I perform single read runs and still get both index sequences? *

7. Can I run both single read and paired-end recipes with dual-indexed libraries? *

8. How does dual indexing work? *

Is the USER enzyme in our NEBNext kits identical in concentration to the standalone product?