HCV抗体 美国BioFront

BioFront Technologies生产高性价比的HCV检测和定量分析试剂与试剂盒。
我们的NS3 ELISA提供了高敏感性、特异性的病毒检测方法：

• 使用我们全新的NS3 ELISA试剂盒可在90分钟内获取HCV感染的定量检测结果！
• 相比RT-PCR程序省时省钱！
• 高度通用的多位点基因型结果！
• 96孔板规格（12 x 8孔）实现精确的试剂控制！
• 经证明的质量，已发表的结果！

我们的单克隆抗体的敏感性和特异性是无与伦比的：

• 节约成本
• 经过IF、Western blot、TCID50、ELISA和FACS试验验证的性能！
• 经证明的质量，已发表的结果！

Clone:  2E3

Volume:  100 ul (1 ug/ul)

Specificity: Recognizes the helicase domain of JFH-1 NS3 (genotype 2a)

Cross-reactivity:  Cross-reactive against genotype 1a 

Form: Protein A purified

Origin: Mouse

Isotype: IgG1

Buffer/Composition: 1X PBS pH 7.2; 0.1% sodium azide (NaN₃)

Storage: Short Term (<6 months): 4℃. 

Long Term (up to 1 year): -20℃ (avoid repeated freeze/thaw)

Applications: Western, IF, IP, FACS, and TCID50 assay

Working Dilutions: TCID50- 1:1000-1:5000, Western Blot- 1:1000-1:5000

IF- 1:1000, FACS- 1:500

References:

(1) Jirasko et al. PLoS Pathog. 2010 Dec 16;6(12):e1001233.

(2) Backes et al. J Virol. 2010 Jun;84(11):5775-89

(3) Kaul et al. PLoS Pathog. 2009 Aug;5(8):e1000546

(4) Yang et al. J Virol. 2008 Jun;82(11):5269-78.

Cell lysate run on 12% SDS-PAGE and transferred to PVDF.  Primary antibody incubation performed for 1-hour at room temperature (overnight incubations also suitable at 4 degrees Celsius).

REFERENCES:

Paul D, Hoppe S, Saher G, Krijnse-Locker J, Bartenschlager R. Morphological and biochemical characterization of the membranous hepatitis C virus replication compartment.  J Virol. 2013 Oct;87(19):10612-27.

Friesland M, Mingorance L, Chung J, Chisari FV, Gastaminza P. Sigma-1 receptor regulates early steps of viral RNA replication at the onset of hepatitis C virus infection. J Virol. 2013 Jun;87(11):6377-90.

Fridell RA, Valera L, Qiu D, Kirk MJ, Wang C, Gao M. Intragenic complementation of hepatitis C virus NS RNA replication-defective alleles. J Virol. 2013 Feb;87(4):2320-9.

Wu X, Robotham JM, Lee E, Dalton S, Kneteman NM, Gilbert DM, Tang H. Productive Hepatitis C Virus Infection of Stem Cell-Derived Hepatocytes Reveals a Critical Transition to Viral Permissiveness during Differentiation.  PLoS Pathog 2012; 8 (4).

Nag A, Robotham JM, Tang H. Suppression of Viral RNA Binding and the Assembly of Infectious Hepatitis C Virus Particles In Vitro by Cyclophilin Inhibitors. J Virol. 2012 Dec;86(23):12616-24.

Kenworthy R, Lambert D, Yang F, Wang N, Chen Z, Zhu H, Zhu F, Liu C, Li K, Tang H. Short-hairpin RNAs delivered by lentiviral vector transduction trigger RIG-I-mediated IFN activation.  Nucleic Acids Res. 2009 Oct;37(19):6587-99.

• Item #: HCV-2E3
   

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