Mouse Monoclonal Antibody to HA-tag
General Information |
Immunogen: | HA peptide conjugated with KLH |
Clone ID: | Mouse mAb |
Ig Type: | IgG1 |
Applications: | WB , IHC , IP |
Specificity: | Specifically detected HA tag fused protein. |
Formulation: | 0.2µm filtered solution in PBS with 50% glycerol |
Storage: | <-20℃ |
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with peptide YPYDVPDYA conjugated with KLH. The ascites was purified by ion exchange chromatography.
Applications
1) Western blot (1:1000) : This antibody can be used at 2μg with appropriate secondary reagents to detect HA-tagged proteins.
Lane 1, 2: 293T control Lane 3, 4: 293T transfected with HA plasmid M: protein ladder |
Immunoprecipitation (1:500):This antibody can be used at 2μg to precipitate HA-tagged proteins.
Lane 1: HA mAb IP with 293T transfected with HA plasmid Lane2: HA mAb IP with 293T transfected without HA plasmid M: protein ladder |
Immunofluorescence (1:100): This antibody can be used at 2μg to detect HA-tagged proteins.
293T transfected with HA plasmid |
Specificity
Mouse mAb specifically detects over-expressed proteins containing the HA epitope tag.
Storage
Store at -20℃, and stable for one year from the date of shipment.