嗜热菌蛋白酶Thermolysin from Bacillus thermoproteolyticus rokko sigma产品北京原装现货供应_详细资料

产品信息

数量：大量保存条件： −20°C 英文名： Thermolysin from Bacillus thermoproteolyticus rokko 供应商： qcbio CAS号： 9073-78-3 规格： 100MG原装

Synonym:

Protease from Bacillus thermoproteolyticus rokko, Thermophilic-bacterial protease

CAS Number:

9073-78-3

Description

A thermostable (thermophilic) extracellular metalloendopeptidase containing four calcium ions. Cofactors are zinc and calcium. Hydrolyzes protein bonds on the N-terminal side of hydrophobic amino acid residues. The pH optimum is 8.0 and the optimal temperature for activity is 70 °C. Considerably stable from pH 5 to 9.5. Thermolysin has a low cleavage specificity, therefore, it produces a number of short fragments that are suitable for sequencing. Preferential cleavage: X-cleavage-Y-Z where X=any amino acid; Y=Leu, Phe, Ile, Val, Met, Ala; and Z is any amino acid other than Pro. Cleavage N-terminal to Leu is preferred over cleavage of N-terminal to Phe which is preferred over the others. Often used to do limited proteolysis for peptide mapping and studies of protein structure and conformational changes.

2.Contains many extraneous enzymes.

3.One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).

4.Lyophilized powder containing calcium and sodium buffer salts.

Properties

type

Type X

form

lyophilized powder

mol wt

mol wt 34.6 kDa by amino acid sequence

purified by

crystallization

color

tan or off-white

storage temp.

−20°C

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Synonym:	Protease from Bacillus thermoproteolyticus rokko, Thermophilic-bacterial protease
CAS Number:	9073-78-3

	A thermostable (thermophilic) extracellular metalloendopeptidase containing four calcium ions. Cofactors are zinc and calcium. Hydrolyzes protein bonds on the N-terminal side of hydrophobic amino acid residues. The pH optimum is 8.0 and the optimal temperature for activity is 70 °C. Considerably stable from pH 5 to 9.5. Thermolysin has a low cleavage specificity, therefore, it produces a number of short fragments that are suitable for sequencing. Preferential cleavage: X-cleavage-Y-Z where X=any amino acid; Y=Leu, Phe, Ile, Val, Met, Ala; and Z is any amino acid other than Pro. Cleavage N-terminal to Leu is preferred over cleavage of N-terminal to Phe which is preferred over the others. Often used to do limited proteolysis for peptide mapping and studies of protein structure and conformational changes.
	2.Contains many extraneous enzymes.
	3.One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).
	4.Lyophilized powder containing calcium and sodium buffer salts.

type	Type X
form	lyophilized powder
mol wt	mol wt 34.6 kDa by amino acid sequence
purified by	crystallization
color	tan or off-white
storage temp.	−20°C