MTT噻唑兰，SIGMA M5655

噻唑兰 MTT      品Pai：SIGMApowder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% （HPLC）产品编号：M5655
中文名称：噻唑兰 MTT
英文名称：Thiazolyl Blue Tetrazolium Bromide
CAS号：298-93-1
分子式：C18H16BrN5S
分子量：414.32
纯度：≥97.5%
保存温度：2-8 °C
EC号：206-069-5
MDL号：MFCD00011964
性能描述
Application
Use as a colorimetric metabolic activity indicator in cell viability assays.

 
Thiazolyl Blue Tetrazolium Blue （MTT） may be used in measurement of cell proliferation.1,2,3,4,5 MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol ａnd the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent6 ａnd for the detection of NAD.7 ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used.8 MTT is rapidly reduced to the formazan, which chelates with nickel, copper, ａnd cobalt; the cobalt chelate has been used in oxidative systems.9

product line   BioReagent
assay   ≥97.5% （HPLC）
form   powder
mp   195 °C （dec.）（lit.）
solubility   H2O: soluble5 mg/mL
suitability   suitable for cell culture
  suitable for insect cell culture
storage temp.   2-8°C