性 状: | 【产品类型】Rabbit Monoclonal IgG 【反应特异性】Human , Mouse , Rat 【克隆 ID】EP1331Y Beta Tubulin,Beta Tubulin 【用途】WB , IHC , ICC , FC , IP 【产品性状】 【阳性对照】 【产品浓度】 【细胞定位】 【抗原分子量】50 kDa |
质量标准: | 【产品介绍】Microtubules are essential parts in eukaryotic cell structures, transp o r tation, a n d mitosis a n d consists mainly of 2 soluble protein subunits, alpha a n d beta tubulin. Beta-tubulin binds to alpha tubulin to f o r m tubulin heterodimer which is post-translationally modified (1). The tubulin dimer complex binds to GTP a n d assembles onto the positive ends of microtubules. After inc o r p o r ation into the microtubules, bound GTP is hydrolyzed by beta tubulin. The stability of the dimer in the microtubules is depended on presence of beta tubulin, where dimer with GTP bound beta-tubulin is stable to microtubule inc o r p o r ation (2). Mutation on beta-tubulin has been linked to anti-cancer drug resistance in lung cancers (3). Beta Tubulin,Beta Tubulin Recommended Applications WB, IHC, ICC, FC, IP Applications and Recommended Dilution Factors WB: 1:20,000 IHC: 1:250 - 500 ICC: 1:100 - 250 FC: 1:10 IP: 1:50 Species Reactivity * Human, Mouse, Rat * *Cross reactivity determined by western blot only. Beta Tubulin,Beta Tubulin Products Data A. Western blot analysis on HeLa cell lysate using anti- Beta-Tubulin RabMAb (cat. # 1879-1), dilution 1:20,000. B. Immunohistochemical analysis of paraffin-embedded human gastric carcinoma using anti-Beta-Tubulin RabMAb (cat# 1879-1) Beta Tubulin,Beta Tubulin C. Imunofluorescent staining of HeLa cells using anti- Beta-Tubulin RabMAb (cat# 1879-1) Specificity A synthetic peptide corresponding to residues near the C-term of human β-Tubulin was used as immunogen. |
贮 存: | Beta Tubulin,Beta Tubulin 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |