性 状: | 【反应特异性】Human , Mouse , Rat 【克隆 ID】EPR2208Y B-Raf Phospho (pT401),Rabbit Monoclonal 【用途】WB , IP 【抗原分子量】94 kDa |
质量标准: | 【产品介绍】B-Raf, also know as c-Rmil, is a Serine/Threonine protein kinase member of the Raf family which includes Raf-1 a n d A-Raf. B-Raf is involved in the transduction of mitogenic signals from the cell membrane to the nucleus (1). Composed of three conserved region (CR1, CR2, CR3) B-Raf is expressed primarily in the brain a n d in the nervous system (2). It has been observed that MAPK is activated by B-Raf in response to nerve growth fact o r (3). M o r e than 60% of malignant melanomas were found to contain a specific mutation, B-Raf (V599E) the product of which possesses constitutive kinase activity (4). Mutations in B-Raf have also been identified in lung cancer a n d non-Hodgkin lymphoma. B-Raf Phospho (pT401),Rabbit Monoclonal WB: 1:1000 - 5000 ICC: 1:100 – 250 IP: 1:20 B-Raf Phospho (pT401),Rabbit Monoclonal A. Western blot analysis on PC-12 cell lysates using anti-Phospho-B-Raf (p401) RabMAb (cat. #2298-1), 1:5000 dilution. Cells were either (A) untreated (B) treated with TPA. |