性 状: | 【反应特异性】Human , Mouse 【克隆 ID】E38 Apaf-1 Rabbit Antibody,Rabbit Monoclonal 【用途】WB , IHC , ICC 【抗原分子量】130 kDa |
质量标准: | 【产品介绍】Apaf-1, o r apoptotic protease activating fact o r 1, is a human protein homolog of the C. elagans apoptotic regulat o r y protein CED-4 that activates caspase-9 during apoptosis (1). Cytosolic Apaf-1 f o r ms an oligomeric complex with caspase-9 in the presence of cytochrome c a n d dATP, which leads to caspase-9 activation a n d subsequent activation of caspase-3 (2, 3). Oligomeric Apaf-1 a n d pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains, consequently enhancing the catalytic activity of caspase-9 (4), leading to activation of the caspase signaling cascade a n d apoptosis. Apaf-1 Rabbit Antibody,Rabbit Monoclonal WB: 1:500 IHC: 1:50-1:100 ICC: 1:50-1:100 A. Western blot analysis on HeLa cell lysate using anti-Apaf-1 RabMAb (cat. #1038-1); dilution 1:500. Apaf-1 Rabbit Antibody,Rabbit Monoclonal B. Immunohistochemical analysis of paraffin-embedded stomach adenocarcinoma using anti-Apaf-1 RabMAb (cat. #1038-1). |